Photo-transformation of microplastics and its toxicity to Caco-2 cells.
Photo-transformation of microplastics and its toxicity to Caco-2 cells.
复制标题
微塑料的光转化及其对 Caco-2 细胞的毒性。
DOI：
10.1016/j.scitotenv.2021.150954
复制
发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Xuetao Guo
中科院分区：
文献类型：
--
作者：
Xiaoqin Yu;Mengfan Lang;Daofen Huang;Chengfang Yang;Zhuozhi Ouyang;Xuetao Guo
近年来，微塑料（MPs）污染作为一个全球性的环境问题，受到世界各国的广泛关注。然而，关于议员对人类健康影响的研究仍然有限。在本研究中，我们研究了聚苯乙烯微塑料（PS-MP）在紫外线下的光转化行为及其对 Caco-2 细胞的毒性。我们的结果表明，PS-MPs 的表面因光而变得粗糙，并出现裂纹和凹坑。紫外可见光谱表明该过程中可能存在苯环的打开和羰基的形成。基于FTIR和2D-COS分析，我们观察到羰基和羟基的形成，初步确定PS-MP的光转化顺序为698（C单键H）> 752（C单键H）> 1030（C单键O）> 3645（OH/OOH）> 1740（C双键O）。 XPS表明PS-MPs的光转化是含碳官能团逐渐部分转化为含氧官能团的过程。最后，毒性结果表明，随着PS-MPs浓度的增加和光照射时间的延长，Caco-2细胞的存活率逐渐下降，细胞膜的完整性被破坏。细胞毒性的增加至少可以部分解释为含氧官能团的毒性大于含碳官能团的毒性，但这些官能团如何影响细胞的细胞毒性仍需要未来持续的研究。这项研究可以为理解PS-MPs在环境中的环境行为和生态效应提供新的见解。
In recent years, microplastics (MPs) pollution, as a global environmental problem, has been widely concerned by countries all over the world. However, the research on the impact of MPs on human health is still limited. In this study, we studied the photo-transformation behavior of polystyrene microplastics (PS-MPs) under ultraviolet light and its toxicity to Caco-2 cells. Our results showed that the surface of PS-MPs was roughened by light, and cracks and pits appeared. UV–vis spectra showed that the opening of phenyl ring and the formation of carbonyl group might exist in this process. Based on FTIR and 2D-COS analysis, we observed the formation of carbonyl group and hydroxyl group, and preliminarily determined that the order of photo-transformation of PS-MPs was 698 (Csingle bondH) > 752 (Csingle bondH) > 1030 (Csingle bondO) > 3645 (OH/OOH) > 1740 (Cdouble bondO). XPS showed that the photo-transformation of PS-MPs was a process in which carbon-containing functional groups were gradually partially transformed into oxygen-containing functional groups. Finally, the toxicity results showed that with the increase of PS-MPs concentration and the extension of light irradiation time, the survival rate of Caco-2 cells gradually decreased and the integrity of cell membrane was destroyed. The increased cytotoxicity can be explained at least in part by the fact that the toxicity of oxygen-containing functional groups is greater than that of carbon-containing functional groups, but how these functional groups affect the cytotoxicity of cells still needs sustained research in the future. This study can provide new insights for understanding the environmental behavior and ecological effects of PS-MPs in the environment.