Innate Immune Genes Including a Mucin-Like Gene, mul-1, Induced by Ionizing Radiation in Caenorhabditis elegans
Innate Immune Genes Including a Mucin-Like Gene, mul-1, Induced by Ionizing Radiation in Caenorhabditis elegans
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DOI：
10.1667/rr2989.1
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发表时间：
2012-10
影响因子：
8.9
通讯作者：
Takafumi Kimura;T. Takanami;T. Sakashita;S. Wada;Y. Kobayashi;A. Higashitani
中科院分区：
文献类型：
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作者：
Takafumi Kimura;T. Takanami;T. Sakashita;S. Wada;Y. Kobayashi;A. Higashitani
辐射对肠道的影响已经研究了一百多年。然而，这个器官是否对电离辐射有特定的防御机制仍不清楚。铜绿假单胞菌（PA 14）感染秀丽隐杆线虫诱导天然免疫应答基因上调。在这里，我们发现电离辐射暴露也诱导某些先天性免疫应答基因，如F49 F1.6（称为穆尔-1），clec-4，clec-67，lys-1和lys-2在肠道中。此外，在PA 14草坪板上播种前用电离辐射预处理显著提高了线虫的存活率。我们还研究了穆尔-1在电离辐射下的转录途径。穆尔-1基因的诱导高度依赖于ELT-2转录因子和p38 MAPK。此外，胰岛素/IGF-1信号通路可增强该基因的诱导。穆尔-1基因表现出与DNA损伤反应基因ced-13不同的诱导模式，这意味着该基因的表达可能是辐射的间接效应。穆尔-1基因的沉默导致电离辐射治疗后生长迟缓。我们描述的交叉耐受性之间的反应，辐射暴露和先天免疫系统。
The effect of radiation on the intestine has been studied for more than one hundred years. It remains unclear, however, whether this organ uses specific defensive mechanisms against ionizing radiation. The infection with Pseudomonas aeruginosa (PA14) in Caenorhabditis elegans induces up-regulation of innate immune response genes. Here, we found that exposure to ionizing radiation also induces certain innate immune response genes such as F49F1.6 (termed mul-1), clec-4, clec-67, lys-1 and lys-2 in the intestine. Moreover, pre-treatment with ionizing radiation before seeding on PA14 lawn plate significantly increased survival rate in the nematode. We also studied transcription pathway of the mul-1 in response to ionizing radiation. Induction of mul-1 gene was highly dependent on the ELT-2 transcription factor and p38 MAPK. Moreover, the insulin/IGF-1 signal pathway works to enhance induction of this gene. The mul-1 gene showed a different induction pattern from the DNA damage response gene, ced-13, which implies that the expression of this gene might be triggered as an indirect effect of radiation. Silencing of the mul-1 gene led to growth retardation after treatment with ionizing radiation. We describe the cross-tolerance between the response to radiation exposure and the innate immune system.