Monitoring the expression profiles of doxorubicin-induced and doxorubicin-resistant cancer cells by cDNA microarray.
Monitoring the expression profiles of doxorubicin-induced and doxorubicin-resistant cancer cells by cDNA microarray.
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发表时间：
2000-08
期刊：
影响因子：
11.2
通讯作者：
K. Kudoh;M. Ramanna;R. Ravatn;A. Elkahloun;M. Bittner;P. Meltzer;J. Trent;W. Dalton;K. Chin
中科院分区：
文献类型：
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作者：
K. Kudoh;M. Ramanna;R. Ravatn;A. Elkahloun;M. Bittner;P. Meltzer;J. Trent;W. Dalton;K. Chin
癌症的耐药性是成功进行化疗的主要障碍。暴露在抗肿瘤药物下的癌细胞可能会被直接诱导表达一组可能产生耐药性的基因，从而使一些细胞逃脱杀戮，形成复发的耐药肿瘤。或者，一些癌细胞可能表达一系列可能产生内在耐药的基因，而暴露于细胞毒性药物对形成复发肿瘤的这些细胞的生存具有选择作用。我们已经使用基因芯片监测了被阿霉素瞬时处理或被选择为对阿霉素耐药的MCF-7细胞的表达谱。我们的结果表明，阿霉素的瞬时治疗以时间依赖的方式改变了一组不同的基因的表达。诱导基因的一个子集也被发现在被选为对阿霉素耐药的细胞中结构性过度表达。这组不同的重叠基因可能代表了癌细胞中阿霉素诱导的基因表达和耐药性的特征。我们的研究证明了利用基因芯片技术获取肿瘤细胞中潜在的抗癌药物的分子图谱或指纹图谱的可行性，这可能会对耐药机制有进一步的了解，并提出替代治疗方法。
Drug resistance in cancer is a major obstacle to successful chemotherapy. Cancer cells exposed to antitumor drugs may be directly induced to express a subset of genes that could confer resistance, thus allowing some cells to escape killing and form the relapsed resistant tumor. Alternatively, some cancer cells may be expressing an array of genes that could confer intrinsic resistance, and exposure to cytotoxic drugs select for the survival of these cells that form the relapsed tumor. We have used cDNA microarray to monitor the expression profiles of MCF-7 cells that are either transiently treated with doxorubicin or selected for resistance to doxorubicin. Our results showed that transient treatment with doxorubicin altered the expression of a diverse group of genes in a time-dependent manner. A subset of the induced genes was also found to be constitutively overexpressed in cells selected for resistance to doxorubicin. This distinct set of overlapping genes may represent the signature profile of doxorubicin-induced gene expression and resistance in cancer cells. Our studies demonstrate the feasibility of obtaining potential molecular profile or fingerprint of anticancer drugs in cancer cells by cDNA microarray, which might yield further insights into the mechanisms of drug resistance and suggest alternative methods of treatment.