Initial studies of selenium speciation in Brassica juncea by LC with ICPMS and ES-MS detection: an approach for phytoremediation studies.
Initial studies of selenium speciation in Brassica juncea by LC with ICPMS and ES-MS detection: an approach for phytoremediation studies.
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DOI：
10.1021/ac0106804
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发表时间：
2002
影响因子：
7.4
通讯作者：
M. Montes-Bayón;E. Yanes;C. A. Ponce de León;K. Jayasimhulu;A. Stalcup;J. Shann;J. Caruso
中科院分区：
文献类型：
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作者：
M. Montes-Bayón;E. Yanes;C. A. Ponce de León;K. Jayasimhulu;A. Stalcup;J. Shann;J. Caruso
各种芸苔属物种积累硒达数千ppm。这表明其中一些作为硒植物修复的候选人。芥菜（Brassica juncea）（印度芥菜）通过在水培条件下以亚硒酸钠作为硒源生长来积累硒，导致在植物的各个部分中硒积累高达数百ppm。到目前为止，很少有硒形态的研究已经在植物中进行，与大多数研究报告总硒浓度在不同部位的植物。通过几种消化/提取程序，包括使用HCl，Tris-HCl缓冲液，和酶水解（使用蛋白酶K和蛋白酶XIV）的硒物种提取进行了评价。用蛋白酶K获得最佳提取（提取植物中存在的总硒的约75%）。一些由植物产生的物质，如硒代甲硫氨酸，可以通过RP-HPLC-ICPMS在ppb水平上鉴定，因为标准品很容易获得。其他需要进一步表征ES-MS。酶水解释放主要是硒-蛋氨酸从芥菜叶，虽然其他含硒物种也可以观察到HPLC-ICPMS。在这个初步的研究中，可能的识别（ES-MS）的一个小的色谱峰含有硒-S桥硒氨基酸的结构类似于胱氨酸的建议。
Various Brassica species accumulate Se into the thousands of ppm. This suggests some of them as candidates for Se phytoremediation. Brassica juncea (Indian mustard) was used to accumulate selenium by growing with sodium selenite as the selenium source under hydroponic conditions resulting in Se accumulation of up to hundreds of ppm in various parts of the plant. To date, few selenium speciation studies have been done in plants, with most studies reporting total selenium concentration in various parts of the plant. Se species extraction was evaluated by several digestion/extraction procedures, including the use of HCl, Tris-HCl buffer, and enzymatic hydrolysis (using proteinase K and protease XIV). The best extraction was obtained with proteinase K (extracting approximately 75% of the total Se present in the plant). Some of the species produced by the plant, such as selenomethionine, can be identified at ppb levels by RP-HPLC-ICPMS, since standards are readily available. Others needed to be further characterized by ES-MS. Enzymatic hydrolysis releases mostly Se-methionine from juncea leaves, although other Se-containing species can also be observed by HPLC-ICPMS. In this initial study, the possible identification (by ES-MS) of a small chromatographic peak containing a Se-S bridged seleno amino acid with a structure similar to cystine is suggested.