Evidence for a potent nucleating factor in the gallbladder bile of patients with cholesterol gallstones
Evidence for a potent nucleating factor in the gallbladder bile of patients with cholesterol gallstones
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胆固醇胆结石患者胆囊胆汁中存在有效成核因子的证据
DOI：
10.1016/0016-5085(83)90428-6
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发表时间：
1983
期刊：
影响因子：
29.4
通讯作者：
S. Strasberg
中科院分区：
文献类型：
--
作者：
M. Burnstein;R. G. Ilson;C. Petrunka;R. D. Taylor;S. Strasberg
进行了一项研究，以确定从胆固醇结石患者中获得的胆囊胆汁的快速成核时间是否是由于胆囊添加成核剂或去除抗成核剂。将正常对照组（对照胆汁）和胆结石患者（异常胆汁）的胆囊胆汁的各向同性相以50：50（体积/体积）混合，并测定混合物和母体胆汁的成核时间。该混合物具有快速的成核时间，类似于来自胆结石患者的胆囊胆汁，表明异常胆汁中存在成核因子。然后使用异常胆汁比例降低的混合物进行实验。这些研究表明，成核剂是有效的。结果不是由于胆固醇饱和度或总脂质浓度的变化。第一项研究得出的结论得到了第二组类似实验的支持，在第二组实验中，胆结石患者的肝胆汁与他们自己的胆囊胆汁混合。还发现，通过XM-300 Amicon过滤器过滤异常胆汁并没有消除其成核能力，表明结果不能用异常胆汁中存在残留微晶来解释。
A study was performed to determine whether the rapid nucleation time of gallbladder bile obtained from patients with cholesterol gallstones was due to the addition of a nucleating agent or the removal of an antinucleating agent by the gallbladder. Isotropic phases of gallbladder bile from normal controls (control bile) and from patients with gallstones (abnormal bile) were mixed 50:50 (vol/vol) and the nucleation times of the mixtures and parent biles were determined. The mixtures had rapid nucleation times, similar to those of the gallbladder bile from gallstone patients, indicating that a nucleating factor was present in the abnormal bile. Experiments were then performed using mixtures in which the proportion of abnormal bile was reduced. These studies showed that the nucleating agent was potent. The results were not due to changes in cholesterol saturation or total lipid concentration. The conclusions reached in the first study were supported in a second set of similar experiments in which hepatic bile from gallstone patients was mixed with their own gallbladder bile. It was also found that filtration of abnormal bile through an XM-300 Amicon filter did not eliminate its nucleating potency, indicating that the results could not be explained by the presence of residual microcrystals in the abnormal bile.