Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation.
Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation.
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DOI：
10.1016/j.devcel.2019.12.007
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发表时间：
2019-12
影响因子：
11.8
通讯作者：
Anqi Wang;Janhavi A. Kolhe;Nathan Gioacchini;Imke Baade;W. Brieher;C. Peterson;B. Freeman
中科院分区：
文献类型：
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作者：
Anqi Wang;Janhavi A. Kolhe;Nathan Gioacchini;Imke Baade;W. Brieher;C. Peterson;B. Freeman
间期细胞内染色体位点的移动对于包括RNA转录和基因组组织在内的许多途径至关重要。然而，重组染色质的机制，以应对这些事件还没有报道。在这里，我们描绘了一个分子伴侣依赖的途径重新定位激活的基因位点在酵母。我们提出的数据支持一个模型，其中两个认证系统动员基因启动子通过动态网络的聚合核肌动蛋白。肌球蛋白马达的转录因子依赖性成核推动基因位点通过肌动蛋白基质，并且通过含ARP的染色质重塑物确保肌动蛋白缔合的保真度。核肌球蛋白的运动活性依赖于Hsp90分子伴侣。热休克蛋白90进一步作出贡献，偏向重塑肌动蛋白相互作用的核小体与非经典组蛋白H2A.Z，从而集中在选择的网站，如转录活性基因的途径。总之，该系统提供了一种快速有效的手段，广泛而有选择地动员染色质位点。
Movement of chromosome sites within interphase cells is critical for numerous pathways including RNA transcription and genome organization. Yet, a mechanism for reorganizing chromatin in response to these events had not been reported. Here, we delineate a molecular chaperone-dependent pathway for relocating activated gene loci in yeast. Our presented data support a model in which a two-authentication system mobilizes a gene promoter through a dynamic network of polymeric nuclear actin. Transcription factor-dependent nucleation of a myosin motor propels the gene locus through the actin matrix, and fidelity of the actin association was ensured by ARP-containing chromatin remodelers. Motor activity of nuclear myosin was dependent on the Hsp90 chaperone. Hsp90 further contributed by biasing the remodeler-actin interaction toward nucleosomes with the non-canonical histone H2A.Z, thereby focusing the pathway on select sites such as transcriptionally active genes. Together, the system provides a rapid and effective means to broadly yet selectively mobilize chromatin sites.