Comparison of Akabane virus isolated from sentinel cattle in Japan
Comparison of Akabane virus isolated from sentinel cattle in Japan
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DOI：
10.1016/j.vetmic.2007.03.020
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发表时间：
2007-09-20
影响因子：
3.3
通讯作者：
Akashi, Hiroomi
中科院分区：
文献类型：
--
作者：
Ogawa, Yohsuke;Fukutomi, Toyoko;Akashi, Hiroomi
感染布尼亚病毒科正布尼亚病毒属赤羽病毒（AKAV）的成年奶牛、母羊和山羊不存在任何临床体征;然而，子宫内感染可能导致流产、早产、死产和先天性畸形，如牛、绵羊和山羊的关节弯曲-积水无脑综合征。相比之下，Iriki菌株，从具有神经体征和脑炎的小牛中分离的AKAV的变体，在实验接种的小牛中引起脑炎。两个AKAV现场分离物，命名为Okayama 2001和Okayama 2004，从哨兵小牛的血液标本中分离，并通过交叉中和试验，S和M RNA片段的遗传分析和小鼠实验性腹腔感染进行表征。虽然在Okayama 2001和Iriki菌株之间建立了遗传关系，但它们的抗原特性不同。Okayama 2001在小鼠中是无毒的，OBE-1菌株也是如此，OBE-1菌株是从流产的牛胎儿中分离的。相反，Okayama 2004与OBE-1菌株在抗原性和遗传学上相关，但在小鼠中具有毒性，与Iriki菌株相似。这些结果表明，分离物的抗原性或致病性突变，并建议AKAV突变频繁，在该领域。虽然已经开发了减毒和灭活疫苗用于疾病预防，但由于变异产生的变异病毒可能会发生疫情。(c)2007 Elsevier B. V.保留所有权利。
Adult cows, ewes, and goats infected with Akabane virus (AKAV) of the genus Orthobunyavirus of the family Bunyaviridae do not present any clinical signs; however, in utero infections may result in abortion, premature birth, stillbirth, and congenital deformities such as arthrogryposis-hydranencephaly syndrome in cattle, sheep, and.goats. In contrast, the Iriki strain, a variant of AKAV isolated from a calf with nervous signs and encephalitis, causes encephalitis in experimentally inoculated calves. Two AKAV field isolates, named Okayama2001 and Okayama2004, were isolated from blood specimens of sentinel calves and characterized by cross-neutralization testing, genetic analyses of the S and M RNA segments, and experimental intraperitoneal infection in mice. Although a genetic relationship was established between Okayama2001 and the Iriki strain, their antigenic characteristics differ. Okayama2001 was avirulent in mice, as was the OBE-1 strain, which was isolated from an aborted bovine fetus. In contrast, Okayama2004 was antigenically and genetically related to the OBE-1 strain, but was virulent in mice, similar to the Iriki strain. These results indicate that the isolates mutated antigenically or pathogenically and suggest that AKAV mutates frequently in the field. Although attenuated and inactivated vaccines have been developed for disease prevention, an outbreak may occur due to variant viruses arising from mutation. (c) 2007 Elsevier B.V. All rights reserved.