Abnormal lipid peroxidation in patients with sleep apnoea
Abnormal lipid peroxidation in patients with sleep apnoea
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DOI：
10.1034/j.1399-3003.2000.16d13.x
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发表时间：
2000-10-01
影响因子：
24.3
通讯作者：
Augustí, AGN
中科院分区：
文献类型：
--
作者：
Barceló, A;Miralles, C;Augustí, AGN
阻塞性睡眠呼吸暂停综合征（OSAS）患者心血管疾病的患病率增加。每次呼吸暂停后动脉氧合的下降和上升可能增加脂质过氧化作用，有助于解释这种相关性。本研究测定了14例男性重度OSAS患者的脂质过氧化反应及持续气道正压通气（CPAP）治疗的效果（59+/-5呼吸暂停.h（-1））（+/-SEM）和13名年龄相似的健康非吸烟男性志愿者进行了研究，患者在诊断时和CPAP治疗后超过1年（>4 h.night（-1））进行研究。整夜禁食后清晨采集静脉血样。在OSAS患者中，还获得了睡眠前和睡眠中的样本。采用连续超离心法分离低密度脂蛋白（LDL）颗粒。氧化水平用硫代巴比妥酸测定法（TBARS）测定，氧化敏感性用滞后期测定法测定。OSAS患者TBARS较高（28.1+/-2.8 vs. 20.0+/-1.8 nmol.丙二醛.mgLDL蛋白（-1），p=0.02）和较短的滞后期值（83.8+/-3.4 vs 99.7+/-3.4 min，p=0.005）。这些差异不是由于患者的吸烟状况所致。同样，这些值在夜间没有显著变化，经CPAP治疗后，滞后期值显著改善（124.9+/-8.5 min; p
The prevalence of cardiovascular diseases is increased in patients with the obstructive sleep apnoea syndrome (OSAS), The fall and rise of arterial oxygenation that follows each apnoea may increase lipid peroxidation and contributes to explaining this association. In the present study, the authors determined lipid peroxidation in patients with OSAS and the effect of treatment with continuous positive airway pressure (CPAP),Fourteen male patients with severe OSAS (59+/-5 apnoea.h(-1)) (+/-SEM) and 13 healthy nonsmoking, male volunteers of similar age were studied, Patients were studied at diagnosis and after treatment with CPAP for more than 1 yr (>4 h.night(-1)). A venous blood sample was obtained early in the morning after fasting all night. In patients with OSAS, a sample before and during sleep was also obtained. tow density Lipoprotein (LDL) particles were isolated by sequential ultracentrifugation. Their level of oxidation was determined by the thiobarbituric acid assay (TBARs), and their susceptibility to oxidation by the lag phase measurement,Patients with OSAS showed higher TBARs (28.1+/-2.8 versus 20.0+/-1.8 nmol.malondialdehyde.mgLDL protein(-1), p=0.02) and shorter lag phase values (83.8+/-3.4 versus 99.7+/-3.4 min, p=0.005) than controls. These differences were not due to the smoking status of the patient. Likewise, these values did not change significantly throughout the night yet, the lag phase value was significantly improved by treatment with CPAP (124.9+/-8.5 min; p