Role of Sod Gene in Response to Static Magnetic Fields in Pseudomonas aeruginosa
Role of Sod Gene in Response to Static Magnetic Fields in Pseudomonas aeruginosa
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DOI：
10.1007/s00284-017-1264-4
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发表时间：
2017-08-01
影响因子：
2.6
通讯作者：
Landoulsi, Ahmed
中科院分区：
文献类型：
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作者：
Hanini, Raouia;Chatti, Abdelwaheb;Landoulsi, Ahmed
超氧化物歧化酶（SOD）对非电离辐射，如静态电磁场（200 mT）的保护作用已在铜绿假单胞菌的野生型和突变株缺乏胞质Mn-SOD（sodM），Fe-SOD（sodB），或两种SOD（sodMB）进行了研究。我们的研究结果表明，sodM和/或sodB基因的失活增加了铜绿假单胞菌对静磁场（200 mT）诱导的应激的敏感性。此外，我们的研究结果表明，超氧化物歧化酶，过氧化氢酶和过氧化物酶的增强后，暴露于磁场。然而，野生型细胞保持显着较高的抗氧化酶活性比突变株。与野生型相比，突变株中不饱和脂质和脂肪酸氧化降解产生的丙二醛显着增加。结果表明，SOD对电磁场诱导的铜绿假单胞菌应激具有保护作用。
The protective role of superoxide dismutase (SOD) against non-ionizing radiation such as static electromagnetic field (200 mT) has been studied in wild-type and mutant strain of Pseudomonas aeruginosa lacking cytosolic Mn-SOD (sodM), Fe-SOD (sodB), or both SODs (sodMB). Our results showed that inactivation of sodM and/or sodB genes increases the sensitivity of P. aeruginosa toward stress induced by the static magnetic field (200 mT). Furthermore, our results showed an enhancement of SOD, catalase, and peroxidases after exposure to the magnetic field. However, wild-type cells maintained significantly higher activities of antioxidant enzymes than mutant strains. The malondialdehyde produced by the oxidative degradation of unsaturated lipids and fatty acids showed significant increase in mutant strains compared to the wild-type. The overall results showed that the SOD has a protective role against a stress induced by static electromagnetic field in P. aeruginosa.