Stress granule formation in Entamoeba histolytica: cross‐talk between EhMLBP, EhRLE3 reverse transcriptase and polyubiquitinated proteins
Stress granule formation in Entamoeba histolytica: cross‐talk between EhMLBP, EhRLE3 reverse transcriptase and polyubiquitinated proteins
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溶组织内阿米巴中应激颗粒的形成：EhMLBP、EhRLE3 逆转录酶和多泛素化蛋白之间的串扰
DOI：
10.1111/cmi.12273
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发表时间：
2014
影响因子：
3.4
通讯作者：
Ankri S.
中科院分区：
文献类型：
--
作者：
Katz S;Trebicz-Geffen M;Ankri S.
溶组织内阿米巴甲基化LINE结合蛋白（EhMLBP）与甲基化重复DNA结合，是长散布核苷酸元件（LINE）逆转录酶的正调控因子。这种蛋白质通过减少蛋白质聚集来保护滋养体免受热休克。热休克诱导的蛋白质聚集体中EhMLBP和多泛素化蛋白的存在提出了这些蛋白质是否相互作用的问题。免疫共沉淀实验证实了这一假设：在非应激溶组织肠杆菌滋养体的核周区域检测到泛素化蛋白，而在热休克滋养体的核周区域检测到泛素化蛋白，并与EhMLBP共定位于细胞质颗粒中。我们还观察到EhRLE3逆转录酶的过表达诱导EhMLBP表达的上调和这些含EhMLBP颗粒的形成。由于（i）这些在热休克的溶组织肠杆菌滋养体细胞质中的含EhMLBP的颗粒也含有多聚泛素化蛋白和poly（A）+mRNA，（ii）它们的形成被砷酸钠、嘌呤霉素和帕特胺A促进，并被放线菌酮抑制，我们认为这些细胞质含EhMLBP的颗粒是应激颗粒。我们的数据还表明这些颗粒的形成取决于EhMLBP和LINE。
TheEntamoeba histolytica‐methylated LINE‐binding protein (EhMLBP) binds to methylated repetitive DNA and is a positive regulator of a reverse transcriptase of a long interspersed nucleotide element (LINE). This protein protects trophozoites against heat shock by reducing protein aggregation. The presence of EhMLBP and polyubiquitinated proteins in heat shock‐induced protein aggregates raised the question whether these proteins interact. This assumption was confirmed by co‐immunoprecipitation experiments: ubiquitinated proteins were detected in the perinuclear region of non‐stressedE. histolyticatrophozoites, whereas ubiquitinated proteins were detected in the perinuclear region and colocalized with EhMLBP in cytoplasmic granules in heat‐shocked trophozoites. We also observed that overexpression of the reverse transcriptase of EhRLE3 induced the upregulation of EhMLBP expression and the formation of these EhMLBP‐containing granules. Since (i) these EhMLBP‐containing granules in the cytoplasm of heat‐shockedE. histolyticatrophozoites also contain polyubiquitinated proteins and poly(A)+mRNA and (ii) their formation is promoted by sodium arsenate, puromycin, and pateamine A and is inhibited by cycloheximide, we propose that these cytoplasmic EhMLBP‐containing granules are stress granules. Our data also suggest that the formation of these granules is dependent upon EhMLBP and LINE.
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发表时间：
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期刊：
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