Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.
Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.
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DOI：
10.1371/journal.pone.0020081
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ishihama A
中科院分区：
文献类型：
--
作者：
Shimada T;Fujita N;Yamamoto K;Ishihama A
CAMP受体蛋白(cAMP Receptor Protein，cAMP)是无糖条件下碳源利用基因的全球调节因子，是研究最深入的原核转录因子。大肠杆菌基因组上共有195个靶启动子被认为受cAMP结合的CRP的控制。然而，使用新开发的转录因子结合序列基因组SELEX筛选系统，我们总共鉴定了至少254个CRP结合位点。根据它们在大肠杆菌基因组上的位置，我们预测总共至少有183个新的调控靶操纵子，总共有195个已知的调控靶，达到最少的378个启动子作为cAMP-CRP的调控靶。从新鉴定的靶标中筛选出的所有启动子都被LacZ报告基因检测到在CRP的控制之下，这表明基因组SELEX筛选可以高精度地识别CRP靶标。根据新的靶基因的功能，我们得出结论，从碳源的选择性运输，糖酵解-糖异生转换到糖酵解下游的代谢，包括三羧酸(TCA)循环、丙酮酸脱氢酶(PDH)途径和有氧呼吸，CRP在整个过程中起着关键的调节作用。一种独特的调控模式是，在基因间隔区结合的单个相同的CRP分子经常调节两个不同转录的操纵子。
CRP (cAMP receptor protein), the global regulator of genes for carbon source utilization in the absence of glucose, is the best-studied prokaryotic transcription factor. A total of 195 target promoters on the Escherichia coli genome have been proposed to be under the control of cAMP-bound CRP. Using the newly developed Genomic SELEX screening system of transcription factor-binding sequences, however, we have identified a total of at least 254 CRP-binding sites. Based on their location on the E. coli genome, we predict a total of at least 183 novel regulation target operons, altogether with the 195 hitherto known targets, reaching to the minimum of 378 promoters as the regulation targets of cAMP-CRP. All the promoters selected from the newly identified targets and examined by using the lacZ reporter assay were found to be under the control of CRP, indicating that the Genomic SELEX screening allowed to identify the CRP targets with high accuracy. Based on the functions of novel target genes, we conclude that CRP plays a key regulatory role in the whole processes from the selective transport of carbon sources, the glycolysis-gluconeogenesis switching to the metabolisms downstream of glycolysis, including tricarboxylic acid (TCA) cycle, pyruvate dehydrogenase (PDH) pathway and aerobic respiration. One unique regulation mode is that a single and the same CRP molecule bound within intergenic regions often regulates both of divergently transcribed operons.
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影响因子：
3.2
作者：
Bettenbrock, Katja;Sauter, Thomas;Gilles, Ernst-Dieter
通讯作者：
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影响因子：
5.6
作者：
DALDAL, F
通讯作者：
DALDAL, F
影响因子：
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作者：
Brown, Greg;Singer, Alexander;Yakunin, Alexander F.
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影响因子：
9.9
作者：
通讯作者：
--
DOI：
10.1073/pnas.0506687102
发表时间：
2005-12-06
影响因子：
11.1
作者：
Grainger, DC;Hurd, D;Busby, SJW
通讯作者：
Busby, SJW