ISOLATION AND CHARACTERIZATION OF A HUMAN CDNA CLONE ENCODING A NOVEL DNA TOPOISOMERASE-II HOMOLOG FROM HELA-CELLS
ISOLATION AND CHARACTERIZATION OF A HUMAN CDNA CLONE ENCODING A NOVEL DNA TOPOISOMERASE-II HOMOLOG FROM HELA-CELLS
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DOI：
10.1016/0014-5793(90)81520-x
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发表时间：
1990-06-18
期刊：
影响因子：
3.5
通讯作者：
FISHER, LM
中科院分区：
文献类型：
--
作者：
AUSTIN, CA;FISHER, LM
我们从HeLa癌细胞cDNA文库中分离并测序了3个人类DNA拓扑异构酶II （topo II）部分cDNA克隆。两个克隆与HeLa topo II cDNA的内部片段相同。第三个克隆CAA5具有不同的新序列，与HeLa topo II cDNA区域具有显著的核苷酸同源性（62%）和预测肽同源性（70%）。我们的研究结果表明，HeLa细胞至少表达两种同源形式的DNA拓扑异构酶II。新的HeLa topo II同源物与最近在伯基特淋巴瘤和其他细胞系中描述的topo II同工酶有关。
We have isolated and sequenced 3 human DNA topoisomerase II (topo II) partial cDNA clones from a HeLa carcinoma cell cDNA library. Two clones were identical to an internal fragment of HeLa topo II cDNA. The third clone, CAA5, had a different and novel sequence which shared significant nucleotide (62%) and predicted peptide (70%) homologies with a region of the HeLa topo II cDNA. Our results suggest that HeLa cells express at least two homologous forms of DNA topoisomerase II. The new HeLa topo II homologue is discussed in relation to topo II isoenzymes recently described in a Burkitt lymphoma and other cell lines.