Histochemical examination of osteoblastic activity in op/op mice with or without injection of recombinant M-CSF
Histochemical examination of osteoblastic activity in op/op mice with or without injection of recombinant M-CSF
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注射或不注射重组 M-CSF 的 op/op 小鼠成骨细胞活性的组织化学检查
DOI：
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发表时间：
2001
影响因子：
3.3
通讯作者：
H. Ozawa
中科院分区：
文献类型：
--
作者：
I. Nishino;N. Amizuka;H. Ozawa
抽象。由于巨噬细胞集落刺激因子（M-CSF）耗竭导致破骨细胞形成缺陷，骨硬化（OP/OP）小鼠未表现出骨重建。在本研究中，我们研究了有或没有预先注射M-CSF的OP/OP小鼠的胫骨，以确定破骨细胞的形成和随后的骨吸收是否可以激活成骨细胞，这被称为“耦合”现象。在OP/OP小鼠中，不存在破骨细胞，但与生长板软骨相邻的干骺端成骨细胞似乎是活跃的，揭示了强烈的碱性磷酸酶（ALP 3）免疫反应性。因此，初级小梁骨连续延伸至骨干，表明在op/op小鼠中良好地实现了骨建模。与干骺端相比，大多数骨干成骨细胞被扁平化，并显示出弱的ALP 13活性，因此，它们似乎不太活跃。骨桥蛋白（OPN）在初级骨小梁的骨和软骨基质之间的界面上有轻微的定位。相反，在OP/OP小鼠注射M-CSF，抗酒石酸酸性磷酸酶阳性破骨细胞出现，吸收骨干区域的骨小梁。在活跃的破骨细胞附近的骨干成骨细胞呈立方形，并表现出强烈的ALTH免疫反应。骨桥蛋白不仅在骨-软骨界面上表达，而且在破骨细胞下方的骨吸收陷窝中也有表达。这些观察结果表明，骨干成骨细胞的活化似乎与破骨细胞的形成和随后的骨吸收相结合。或者，软骨-骨交界处的干骺端成骨细胞似乎受骨细胞活性的影响较小。
Abstract. Osteopetrotic (op/op) mice do not exhibit bone remodeling because of defective osteoclast formation caused by the depletion of macrophage colony-stimulating factor (M-CSF). In the present study, we investigated tibial bones of op/op mice with or without prior injections of M-CSF to determine whether osteoclast formation and subsequent bone resorption could activate osteoblasts, which is known as a "coupling" phenomenon. In op/op mice, no osteoclasts were present, but the metaphyseal osteoblasts adjacent to the growth plate cartilage seemed to be active, revealing an intense alkaline phosphatase (ALPase) immunoreactivity. Consequently, primary trabecular bones were extended continuously to the diaphysis, indicating that bone modeling is well achieved in op/op mice. In contrast with the metaphysis, most of the diaphyseal osteoblasts were flattened and showed weak ALPase activity, and, as a result, they seemed to be less active. Osteopontin (OPN) was localized slightly at the interface between bone and cartilage matrices of the primary trabeculae. In contrast, in op/op mice injected with M-CSF, tartrate-resistant acid phosphatase-positive osteoclasts appeared, resorbing trabecular bones of the diaphyseal region. The diaphyseal osteoblasts in the vicinity of the active osteoclasts were cuboidal and exhibited strong ALPase immunoreactivity. OPN was observed not only at the bone–cartilage interface, but also significantly on the resorption lacunae beneath the bone-resorbing osteoclasts. These observations indicate that the activation of diaphyseal osteoblasts appears to be coupled with osteoclast formation and subsequent osteoclastic bone resorption. Alternatively, the metaphyseal osteoblasts at the chondro–osseous junction seemed to be less affected by osteoclastic activity.
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集落刺激因子 1 注射可改善骨硬化 (op) 小鼠的骨骼硬化症，但不能治愈。
DOI：
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发表时间：
1995
期刊：
Bone.
影响因子：
--
作者：
Sundquist,KT;Cecchini,MG;MarksJr,SC
通讯作者：
MarksJr,SC
DOI：
10.1016/s0021-9258(18)98430-9
发表时间：
1993-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
F. Ross;J. Chappel;Jose I. AlvarezS;Diane Sanderll;W. Butler;M. Farach-Carson;Keith A. Mintz;P. Robey;Steven L. TeitelbaumS;David A. Chereshll
通讯作者：
F. Ross;J. Chappel;Jose I. AlvarezS;Diane Sanderll;W. Butler;M. Farach-Carson;Keith A. Mintz;P. Robey;Steven L. TeitelbaumS;David A. Chereshll
影响因子：
2.6
作者：
W. Wiktor-Jedrzejczak;E. Urbanowska;S. L. Aukerman;J. Pollard;E. Stanley;P. Ralph;A. Ansari;K. Sell
通讯作者：
W. Wiktor-Jedrzejczak;E. Urbanowska;S. L. Aukerman;J. Pollard;E. Stanley;P. Ralph;A. Ansari;K. Sell
小鼠骨髓中破骨细胞和成骨细胞祖细胞的骨桥蛋白表达：证明其对破骨细胞生成的要求及其在卵巢切除后的增加。
影响因子：
4.8
作者：
Yamate,T;Mocharla,H;Taguchi,Y;Igietseme,JU;Manolagas,SC;Abe,E
通讯作者：
Abe,E