Quantification of anisotropy and orientation in 3D electron microscopy and diffusion tensor imaging in injured rat brain
Quantification of anisotropy and orientation in 3D electron microscopy and diffusion tensor imaging in injured rat brain
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损伤大鼠大脑 3D 电子显微镜和扩散张量成像中各向异性和方向的量化
DOI：
10.1016/j.neuroimage.2018.01.087
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发表时间：
2018
期刊：
影响因子：
5.7
通讯作者：
A. Sierra
中科院分区：
文献类型：
--
作者：
R. Salo;I. Belevich;Eppu Manninen;E. Jokitalo;O. Gröhn;A. Sierra
扩散张量成像（DTI）可无创性地显示灰质和白色物质的显微结构特征。然而，DTI产生的对比度尚未完全理解，需要进一步验证。我们使用连续块面扫描电子显微镜（SBEM）来获取组织度量，即，使用基于三维傅立叶变换（3D-FT）的分析来确定各向异性和取向，以与DTI中的分数各向异性和取向相关。SBEM在介观尺度上产生高分辨率的3D数据，具有良好的细胞膜对比度。我们分析了从假手术和创伤性脑损伤（TBI）大鼠的扣带回、胼胝体和病灶周围皮质中选择的样本。DTI和3D-FT在所有样本中产生的主方向一致。各向异性值显示了类似的模式，相应的DTI和3D-FT参数在假手术和TBI大鼠的变化。虽然在灰质中DTI和3D-FT各向异性值相似，但在白色物质中3D-FT各向异性值始终低于DTI的分数各向异性值。我们还评估了3D-FT分析中分辨率的影响。尽管灰质样本的角度差异很小，但较低分辨率的数据集提供了可靠的结果，允许分析更大的视野。总体而言，3D SBEM允许从组织微观结构角度对扩散成像对比度进行更复杂的验证研究。
Diffusion tensor imaging (DTI) reveals microstructural features of grey and white matter non-invasively. The contrast produced by DTI, however, is not fully understood and requires further validation. We used serial block-face scanning electron microscopy (SBEM) to acquire tissue metrics, i.e., anisotropy and orientation, using three-dimensional Fourier transform-based (3D-FT) analysis, to correlate with fractional anisotropy and orientation in DTI. SBEM produces high-resolution 3D data at the mesoscopic scale with good contrast of cellular membranes. We analysed selected samples from cingulum, corpus callosum, and perilesional cortex of sham-operated and traumatic brain injury (TBI) rats. Principal orientations produced by DTI and 3D-FT in all samples were in good agreement. Anisotropy values showed similar patterns of change in corresponding DTI and 3D-FT parameters in sham-operated and TBI rats. While DTI and 3D-FT anisotropy values were similar in grey matter, 3D-FT anisotropy values were consistently lower than fractional anisotropy values from DTI in white matter. We also evaluated the effect of resolution in 3D-FT analysis. Despite small angular differences in grey matter samples, lower resolution datasets provided reliable results, allowing for analysis of larger fields of view. Overall, 3D SBEM allows for more sophisticated validation studies of diffusion imaging contrast from a tissue microstructural perspective.
影响因子：
4.2
作者：
DIETRICH, WD;ALONSO, O;HALLEY, M
通讯作者：
HALLEY, M
影响因子：
14.5
作者：
Budde, Matthew D.;Janes, Lindsay;Frank, Joseph A.
通讯作者：
Frank, Joseph A.