Recombinant migration inhibitory factor induces nitric oxide synthase in murine macrophages.
Recombinant migration inhibitory factor induces nitric oxide synthase in murine macrophages.
复制标题
DOI：
10.4049/jimmunol.150.5.1908
复制
发表时间：
1993-03
影响因子：
4.4
通讯作者：
F. Cunha;W. Weiser;J. David;D. Moss;S. Moncada;F. Liew
中科院分区：
文献类型：
--
作者：
F. Cunha;W. Weiser;J. David;D. Moss;S. Moncada;F. Liew
A recombinant form of migration inhibitory factor (MIF) obtained from COS-1 cells transfected with a cDNA library from a human T cell hybridoma is able to activate, in a dose-dependent manner, murine macrophages to express nitric oxide (NO) synthase and to produce high levels of NO in vitro. The time course of the induction of NO synthase is similar to that produced by the IFN-gamma. Enzyme activity peaks at 24 h and is undetectable by 72 h. MIF can synergize with IFN-gamma in the induction of NO synthesis, and the induction of NO synthase by both MIF and IFN-gamma is sensitive to inhibition by dexamethasone. However, unlike IFN-gamma-induced NO generation, MIF is sufficient for the induction of the enzyme, does not synergize with LPS, and is highly sensitive to inhibition by transforming growth factor.
