A guide to analysis of cardiac phenotypes in the zebrafish embryo.
A guide to analysis of cardiac phenotypes in the zebrafish embryo.
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DOI：
10.1016/b978-0-12-387036-0.00007-4
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发表时间：
2011
影响因子：
--
通讯作者：
Yelon D
中科院分区：
文献类型：
--
作者：
Miura GI;Yelon D
斑马鱼是研究心脏发生分子机制的理想模式生物，因为胚胎心脏的光学通路和遗传分析的大量机会强有力地结合在一起。越来越多的研究揭示了突变、morpholinos和小分子，它们会导致斑马鱼胚胎中显著的心脏缺陷和血液循环中断。这些缺陷可以由各种各样的起源引起，包括心脏祖细胞的规格或分化缺陷；心管、心腔或房室管形态发生错误或建立正常心功能有问题。广泛的技术库可用于区分这些可能性，从而破译心脏缺陷的根源。在本章中，我们提供了对斑马鱼胚胎中心脏表型表征特别有效的实验策略指南。
The zebrafish is an ideal model organism for investigating the molecular mechanisms underlying cardiogenesis, due to the powerful combination of optical access to the embryonic heart and plentiful opportunities for genetic analysis. A continually increasing number of studies are uncovering mutations, morpholinos, and small molecules that cause striking cardiac defects and disrupt blood circulation in the zebrafish embryo. Such defects can result from a wide variety of origins including defects in the specification or differentiation of cardiac progenitor cells; errors in the morphogenesis of the heart tube, the cardiac chambers, or the atrioventricular canal or problems with establishing proper cardiac function. An extensive arsenal of techniques is available to distinguish between these possibilities and thereby decipher the roots of cardiac defects. In this chapter, we provide a guide to the experimental strategies that are particularly effective for the characterization of cardiac henotypes in the zebrafish embryo.