Neurodevelopmental consequences of gestational exposure (GD14-GD20) to low dose deltamethrin in rats
Neurodevelopmental consequences of gestational exposure (GD14-GD20) to low dose deltamethrin in rats
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DOI：
10.1016/s0304-3940(01)01543-9
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发表时间：
2001-03-16
影响因子：
2.5
通讯作者：
Seth, PK
中科院分区：
文献类型：
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作者：
Aziz, MH;Agrawal, AK;Seth, PK
宫内低水平接触溴氰菊酯(DT)(1 mg/kg体重)的影响在孕14-20天，对出生后6周和12周的大鼠的神经行为、神经化学和免疫组织化学参数进行了研究。DT暴露组大鼠海马区乙酰胆碱酯酶活性显著升高，H-3-奎宁基苯甲酸结合量显著降低，提示胆碱能(毒鼠碱)受体受损。在6周和12周时，学习和记忆能力也显著下降，这与M受体结合的减少直接相关。生长相关蛋白-43是一种存在于轴突生长锥中的神经元特异性蛋白，是神经元分化和突触形成的标志，免疫组织化学和图像分析显示，在DT暴露的两个时间段，其在海马区的表达均异常增加。这些数据表明，在大脑生长发育期，子宫内低水平的DT暴露会对发育中的大脑产生不利影响，这种变化甚至会持续到出生后12周。虽然在12周评估时没有显着的恢复，但在生化和行为参数上仍然存在显着的损害。(C)2001爱思唯尔爱尔兰科学有限公司。保留所有权利。
Effect of low level in utero exposure to deltamethrin (DT) (1 mg/kg wt.) during gestation day 14-20 was studied on selected neurobehavioral, neurochemical, immunohistochemical parameters in rats at 6 and 12 weeks postnatal period. The significant increase in acetylcholinesterase activity and decrease in H-3-quinuclidinyl benzilate binding in the hippocampal region of DT exposed animals, suggesting impairment in cholinergic (muscarinic) receptors. A significant decrease in the learning and memory performances was also observed both at 6 and 12 weeks, which is directly correlated with decrease in muscarinic receptor binding. Immunohistochemistry and image analysis of growth associated protein-43, a neuron specific protein present in axonal growth cone and a marker for neuronal differentiation and synaptogenesis, exhibit aberrant increase in its expression in the hippocampus in DT exposed rats at both time periods. The data suggests that low level exposure to DT in utero during brain growth spurt period adversely affects the developing brain and the changes persist even upto 12 weeks postnatal period in rats. Although there is no significant recovery at 12 weeks assessment but still significant impairment persist on biochemical and behavioural parameters. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.