Reduction of OsFLW7 expression enhanced leaf area and grain production in rice
Reduction of OsFLW7 expression enhanced leaf area and grain production in rice
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减少 OsFLW7 表达可增加水稻的叶面积和籽粒产量
DOI：
10.1016/j.scib.2017.11.013
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发表时间：
2017
期刊：
影响因子：
18.9
通讯作者：
Qian Qian
中科院分区：
文献类型：
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作者：
Xu Jing;Wang Li;Wang Yue-xing;Zeng Da-li;Zhou Meng-yu;Fu Xue;Ye Wei-jun;Hu Jiang;Zhu Li;Ren De-yong;Gao Zhen-yu;Dong Guo-jun;Guo Long-biao;Zhang Guang-heng;Qian Qian
2017中国科学出版社由Elsevier BV和Science China Press出版。增加水稻产量是确保中国粮食安全的重要因素[1]。挖掘产量潜力、寻找对产量有利的基因是现代水稻育种的重要目标。一般而言，控制叶片形态、提高光合效率、调节“库源”关系，可以促进水稻等粮食作物高产育种[2-4]。叶的形态包括从分生组织出现后的长度、宽度和卷曲度，并且由沿着近轴-远轴、腹背和内侧-外侧轴的极性的建立来确定，并且在遗传、激素和环境水平上进行调节[5，6]。水稻叶片形态在水稻亚种和栽培品种间差异很大。影响叶片形状的数量性状位点（QTL）通常对水稻产量具有多效性效应[7-9]，比突变基因更有助于研究叶片形状调控的分子机制及其在水稻育种中的应用。在本研究中，我们使用高产籼稻品种93-11和优质粳稻品种日本晴（NPB）作为亲本菌株，这两个品种在植株形态、叶片大小和籽粒性状上存在显著差异（图S1，在线），并且两个品种都具有良好的遗传背景和完整的基因组测序。在我们之前的研究中，利用93-11与NPB杂交的207个株系的RIL群体检测到4个叶宽QTL，其中包括第7染色体上影响叶宽的主效QTL qFLW 7（图S2，在线）。在本研究中，针对该QTL，我们通过在NPB和93-11遗传背景中使用93- 11和NPB作为qFLW 7供体，产生了近等基因系NIL-FLW 793 - 11和NIL-FLW 7 NPB（图1a和c），
© 2017 Science China Press. Published by Elsevier BV and Science China Press. All rights reserved.Increasing rice production is important to ensure food security in China [1]. Exploring yield potential and identifying genes beneficial to yield are important goals in the modern rice breeding. Generally, controlling leaf morphology, increasing photosynthesis efficiency and modulating the ‘‘sink-source” relationship can promote the breeding of high-yield rice as well as other cereal crops [2–4]. The morphology of the leaf includes length, width and degree of curl after its emergence from the meristem and is determined by the establishment of polarity along the adaxial–abaxial, ventral–dorsal and medial–lateral axes and regulated at genetic, hormonal and environmental levels [5, 6]. Rice leaf morphology varies widely among rice subspecies and cultivars. Quantitative trait loci (QTLs) affecting leaf shape commonly have pleiotropic effects on rice yield [7–9], which are more useful than mutant genes for studying the molecular mechanism of leaf shape regulation and their application to rice breeding. In the present study, we used the high-yielding indica rice cultivar 93–11 and the high-quality japonica rice cultivar Nipponbare (NPB) as parental strains, which differed markedly in plant morphology, leaf size, and grain traits (Fig. S1, online), and both cultivars have well-characterized genetic backgrounds and completely sequenced genomes. In our previous study, four leaf-width QTLs were detected by using a RIL population of 207 lines derived from the cross of 93-11 and NPB, which included the main effect QTL qFLW7 affecting leaf width on chromosome 7 (Fig. S2, online). In this study, aimed at this QTL, we generated near isogenic lines NIL-FLW793-11and NIL-FLW7NPB (Fig. 1a and c), by using 93-11 and NPB as qFLW7 donors in NPB and 93-11 genetic backgrounds,