The therapeutic effect of cytokine-induced killer cells on pancreatic cancer enhanced by dendritic cells pulsed with K-ras mutant peptide.
The therapeutic effect of cytokine-induced killer cells on pancreatic cancer enhanced by dendritic cells pulsed with K-ras mutant peptide.
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K-Ras 突变肽脉冲的树突状细胞增强细胞因子诱导的杀伤细胞对胰腺癌的治疗作用
DOI：
10.1155/2011/649359
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发表时间：
2011
影响因子：
--
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Tan G;Zhang X;Feng H;Luo H;Wang Z
目标。本研究旨在探讨与K-ras-DC共培养的CIK细胞对胰腺癌细胞株Panc-1(K-ras+)和SW1990(K-ras−)的杀伤作用。方法：研究方法。在干扰素-γ、IL-2和抗CD3单抗诱导下，K-ras-DCCKs由k-ras-DC与CIK共培养获得。用流式细胞仪检测表面标志物。用酶联免疫吸附试验检测干扰素-γ、IL-12、CCL19、CCL22。用~3H-TdR掺入法检测不同细胞因子的增殖情况。用125IUdR检测k-ras-DCCK细胞和CTL的杀伤活性。结果。K-ras-DCCIKs高表达CD3+CD56+和CD3+CD8+。培养上清液中干扰素-γ、IL-12、CCL19和CCL22均显著高于DCCIk和CIK2组。K-ras-DCCIK细胞的杀伤率高于CIK细胞和CTL细胞。K-ras-DC诱导的CTL仅对PANC-1细胞有抑制作用。结论。K-ras-DC可促进CIK细胞的增殖，增强对胰腺癌细胞的杀伤作用。K-ras-DC诱导的CTL仅对PANC-1细胞有抑制作用。在本研究中，K-ras-DCCIKs对PANC-1细胞也表现出特异性的抑制作用，其抑瘤率与CTL几乎相同，其总抑瘤率高于CTL。
Objective. This study is to investigate the role of the CIKs cocultured with K-ras-DCs in killing of pancreatic cancer cell lines, PANC-1 (K-ras+) and SW1990 (K-ras−). Methods. CIKs induced by IFN-γ, IL-2, and anti-CD3 monoantibody, K-ras-DCCIKs obtained by cocultivation of k-ras-DCs and CIKs. Surface markers examined by FACS. IFN-γ IL-12 ,CCL19 and CCL22 detected by ELISA. Proliferation of various CIKs tested via 3H-TdR. Killing activities of k-ras-DCCIKs and CTLs examined with 125IUdR. Results. CD3+CD56+ and CD3+CD8+ were highly expressed by K-ras-DCCIKs. In its supernatant, IFN-γ, IL-12, CCL19 and CCL22 were significantly higher than those in DCCIK and CIK. The killing rate of K-ras-DCCIK was greater than those of CIK and CTL. CTL induced by K-ras-DCs only inhibited the PANC-1 cells. Conclusions. The k-ras-DC can enhance CIK's proliferation and increase the killing effect on pancreatic cancer cell. The CTLs induced by K-ras-DC can only inhibit PANC-1 cells. In this study, K-ras-DCCIKs also show the specific inhibition to PANC-1 cells, their tumor suppression is almost same with the CTLs, their total tumor inhibitory efficiency is higher than that of the CTLs.
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DOI：
10.1084/jem.174.1.139
发表时间：
1991-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Schmidt-Wolf IG;Negrin RS;Kiem HP;Blume KG;Weissman IL
通讯作者：
Weissman IL
影响因子：
10.1
作者：
Introna, Martino;Borleri, Gianmaria;Rambaldi, Alessandro
通讯作者：
Rambaldi, Alessandro
影响因子：
2.6
作者：
Franceschetti, Marta;Pievani, Alice;Introna, Martino
通讯作者：
Introna, Martino
影响因子：
3.7
作者：
Hashimoto Y;Loftis DW;Adams JC
通讯作者：
Adams JC
影响因子：
11.2
作者：
Gobert, Michael;Treilleux, Isabelle;Menetrier-Caux, Christine
通讯作者：
Menetrier-Caux, Christine