Immunolocalization of (1,4)-β-galactan in tension wood fibers of poplar
Immunolocalization of (1,4)-β-galactan in tension wood fibers of poplar
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DOI：
10.1093/treephys/28.8.1263
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发表时间：
2008-08-01
期刊：
影响因子：
4
通讯作者：
Arend, Matthias
中科院分区：
文献类型：
--
作者：
Arend, Matthias
研究了杨树（Populus trichocarpa Torr）木细胞中(1,4)- β -半乳聚糖的发生与分布。& a . Gray x . P. koreana Rehder)研究了这种碳水化合物表位特异性的单克隆抗体（LM5）的免疫标记。免疫荧光显微镜显示LM5抗体与张力木纤维的特异性结合，表明这种木细胞类型的细胞壁中特异性存在(1,4)- β -半乳聚糖。荧光显微镜和免疫金电镜观察结果显示，LM5抗体的结合主要局限于凝胶g层和次级细胞壁之间的狭窄细胞壁区域。这种标记模式强烈表明(1,4)- β -半乳聚糖在g层和次级细胞壁交联中的作用。此外，张力木材中(1,4)- β -半乳聚糖的独家定位强化了这种碳水化合物表位可以被认为是机械应力树木中反应木材形成的高度特异性市场的观点。
The occurrence and distribution of (1,4)-beta-galactan in wood cells of poplar (Populus trichocarpa Torr. & A. Gray x P. koreana Rehder) were studied by immunolabeling with a monoclonal antibody (LM5) specific to this carbohydrate epitope. Immunofluorescence microscopy revealed exclusive binding of the LM5 antibody to tension wood fibers, indicating the specific presence of(1,4)-beta-galactan in cell walls of this wood cell type. Higher magnifications achieved with the fluorescence microscope and additional immunogold electron microscopy showed that the binding of the LM5 antibody was mainly restricted to a narrow cell wall area between the gelatinous G-layer and the secondary cell wall. This labeling pattern strongly suggests a role of (1,4)-beta-galactan in cross-linking the G-layer and secondary cell wall. Furthermore, the exclusive localization of (1, 4)-beta-galactan in tension wood strengthens the view that this carbohydrate epitope can be considered a highly specific market of reaction wood formation in mechanically stressed trees.