In vivo 3D analysis of systemic effects after local heavy-ion beam irradiation in an animal model.
In vivo 3D analysis of systemic effects after local heavy-ion beam irradiation in an animal model.
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DOI：
10.1038/srep28691
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发表时间：
2016-06-27
影响因子：
4.6
通讯作者：
Mitani H
中科院分区：
文献类型：
--
作者：
Nagata K;Hashimoto C;Watanabe-Asaka T;Itoh K;Yasuda T;Ohta K;Oonishi H;Igarashi K;Suzuki M;Funayama T;Kobayashi Y;Nishimaki T;Katsumura T;Oota H;Ogawa M;Oga A;Ikemoto K;Itoh H;Kutsuna N;Oda S;Mitani H
放射治疗被广泛应用于癌症治疗。除了在受照区域内诱发效应外，辐射还可对靠近受照区域和远离受照区域的组织产生效应。日本青竹是一种小型硬骨鱼，也是评估辐射环境影响的模式生物。在这项研究中，我们使用美国国家量子和放射科学与技术研究所的辐射系统，对青竹成人进行了低能碳离子(26.7MeV/u)照射，照射深度约为2.2MeV mm，距离人体表面约2.2MeV mm。我们利用全身连续切片对照射引起的全身改变进行了组织学评估，并进行了心率分析。受照射侧的组织有严重损伤的迹象，与辐射剂量相对应。肾脏切片的3D重建分析显示，肾脏体积和血细胞质量沿照射区域减少，反映了碳束照射造成的损伤的精确定位。腹照射鱼和背照射鱼的鳃中均可观察到毛细血管动脉瘤，提示全身照射效应。本研究为进一步研究局部照射区域以外的照射效应提供了体内模型。
Radiotherapy is widely used in cancer treatment. In addition to inducing effects in the irradiated area, irradiation may induce effects on tissues close to and distant from the irradiated area. Japanese medaka, Oryzias latipes, is a small teleost fish and a model organism for evaluating the environmental effects of radiation. In this study, we applied low-energy carbon-ion (26.7 MeV/u) irradiation to adult medaka to a depth of approximately 2.2 mm from the body surface using an irradiation system at the National Institutes for Quantum and Radiological Science and Technology. We histologically evaluated the systemic alterations induced by irradiation using serial sections of the whole body, and conducted a heart rate analysis. Tissues from the irradiated side showed signs of serious injury that corresponded with the radiation dose. A 3D reconstruction analysis of the kidney sections showed reductions in the kidney volume and blood cell mass along the irradiated area, reflecting the precise localization of the injuries caused by carbon-beam irradiation. Capillary aneurysms were observed in the gill in both ventrally and dorsally irradiated fish, suggesting systemic irradiation effects. The present study provides an in vivo model for further investigation of the effects of irradiation beyond the locally irradiated area.