TYPING HEPATITIS-B VIRUS BY HOMOLOGY IN NUCLEOTIDE-SEQUENCE - COMPARISON OF SURFACE-ANTIGEN SUBTYPES
TYPING HEPATITIS-B VIRUS BY HOMOLOGY IN NUCLEOTIDE-SEQUENCE - COMPARISON OF SURFACE-ANTIGEN SUBTYPES
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DOI：
10.1099/0022-1317-69-10-2575
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发表时间：
1988-10-01
影响因子：
3.8
通讯作者：
MAYUMI, M
中科院分区：
文献类型：
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作者：
OKAMOTO, H;TSUDA, F;MAYUMI, M
从中国大陆、日本冲绳县和印度尼西亚的无症状携带者的血浆中克隆了3株adw亚型的乙肝病毒基因组DNA，测定了它们的全核苷酸序列。这三个序列都有3215个碱基对，序列差异只有3.9%到5.6%。然而，将这些分离株与报道的来自美国携带者的两个相同亚型的乙肝病毒基因组序列进行比较时，差异更大(8.3~9.3%)，其程度与adw亚型和异型亚型(如adr、ayw或ayr)的核苷酸差异相当。总共18个不同亚型的乙肝病毒基因组，包括这里描述的3个，以前报道的10个和5个未发表的，根据核苷酸序列的组间差异8%或更大被分成4组：A组(2个ADW基因组)，B组(4个ADW基因组)，C组(3个ADW基因组，4个ADR基因组和1个AYR基因组)和D组(4个AYW基因组)。因此，HBVadw亚型的9个基因组被分成三组，它们的序列差异很大。这些结果表明，包膜多肽的四种主要抗原性亚型并不能真实反映乙肝病毒的基因变异。A.fwdarw可以引起d到y以及w到r的亚型改变。S基因第365位和第479位核苷酸的G点突变支持这一观点。
The complete nucleotide sequences of the DNA of three hepatitis B virus (HBV) genomes of subtype adw, cloned from plasma samples of asymptomatic carriers living in the mainland and Okinawa Prefecture of Japan and Indonesia were determined. All three comprised 3215 bp and differed in sequence by only 3.9 to 5.6%. When these isolates were compared with the reported sequences of two HBV genomes of the same subtype derived from American carriers, however, the differences were greater (8.3 to 9.3%) to an extent comparable with the nucleotide divergence between an HBV genome of subtype adw and that of a heterotypic subtype, such as adr, ayw or ayr. A total of 18 HBV genomes of various subtypes, including the three described here, 10 reported previously and five unpublished ones, were classified into four groups based on an inter-group divergence in nucleotide sequence of 8% or greater: group A (two adw genomes), group B (four adw), group C (three adw, four adr and one ayr) and group D (four ayw). Thus, the nine genomes of HBV subtype adw were distributed into three groups with considerably different sequences. These results indicate that the four major antigenically defined subtypes of envelope polypeptide do not reflect true genotypic variation of HBV. The fact that d to y, as well as w to r, subtypic change can be induced by an A .fwdarw. G point mutation at nucleotides 365 and 479 in the S gene, respectively, supports this view.