Indefinite and bidirectional near-infrared nanocrystal photoswitching
Indefinite and bidirectional near-infrared nanocrystal photoswitching
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DOI：
10.1038/s41586-023-06076-7
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发表时间：
2022-09
期刊：
影响因子：
64.8
通讯作者：
Changhwan Lee;Emma Z. Xu;K. Kwock;Ayelet Teitelboim;Yawei Liu;H. Park;Benedikt Ursprung;Mark E. Ziffer;Yuzuka Karube;N. Fardian-Melamed;C. Pedroso;Jongwoo Kim;Stefanie D. Pritzl;S. Nam;Theobald Lohmueller;J. Owen;P. Ercius;Y. Suh;B. Cohen;E. Chan;P. Schuck
中科院分区：
文献类型：
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作者：
Changhwan Lee;Emma Z. Xu;K. Kwock;Ayelet Teitelboim;Yawei Liu;H. Park;Benedikt Ursprung;Mark E. Ziffer;Yuzuka Karube;N. Fardian-Melamed;C. Pedroso;Jongwoo Kim;Stefanie D. Pritzl;S. Nam;Theobald Lohmueller;J. Owen;P. Ercius;Y. Suh;B. Cohen;E. Chan;P. Schuck
其发光可以通过光学刺激转换的材料驱动技术，范围从超分辨率成像、纳米光子学和光学数据存储到靶向药理学、光遗传学和化学反应性。这些光可切换探针，包括有机荧光团和蛋白质，可能易于光降解，并且通常在紫外或可见光谱区域中操作。胶体无机纳米颗粒可以提供改进的稳定性，但是据我们所知，在这样的系统中还没有报道双向切换发射的能力，特别是用近红外（NIR）光。在这里，我们展示了雪崩纳米颗粒（ANP）的双向近红外光开关，显示了使用NIR-I和NIR-II光谱区域中的光触发器对上转换发射的完全光学控制，可用于地下成像。采用单步光暗化，，-和光增亮，，-，我们证明了无限期的光开关的个别纳米粒子（超过1,000个周期，7小时）在环境或水性条件下，没有可测量的光降解。的光开关机制的关键步骤阐明建模和测量的光子雪崩特性的单个ANP在明亮和黑暗的状态。ANP的无限制的可逆光开关使得能够实现ANP的无限期二维和三维多级光学图案化，以及具有亚微米定位超分辨率的光学纳米显微镜，其允许我们区分紧密堆积的簇内的单个ANP。
Materials whose luminescence can be switched by optical stimulation drive technologies ranging from superresolution imaging, , –, nanophotonics, and optical data storage,, to targeted pharmacology, optogenetics, and chemical reactivity. These photoswitchable probes, including organic fluorophores and proteins, can be prone to photodegradation and often operate in the ultraviolet or visible spectral regions. Colloidal inorganic nanoparticles,can offer improved stability, but the ability to switch emission bidirectionally, particularly with near-infrared (NIR) light, has not, to our knowledge, been reported in such systems. Here, we present two-way, NIR photoswitching of avalanching nanoparticles (ANPs), showing full optical control of upconverted emission using phototriggers in the NIR-I and NIR-II spectral regions useful for subsurface imaging. Employing single-step photodarkening, , –and photobrightening,, –, we demonstrate indefinite photoswitching of individual nanoparticles (more than 1,000 cycles over 7 h) in ambient or aqueous conditions without measurable photodegradation. Critical steps of the photoswitching mechanism are elucidated by modelling and by measuring the photon avalanche properties of single ANPs in both bright and dark states. Unlimited, reversible photoswitching of ANPs enables indefinitely rewritable two-dimensional and three-dimensional multilevel optical patterning of ANPs, as well as optical nanoscopy with sub-Å localization superresolution that allows us to distinguish individual ANPs within tightly packed clusters.