Responses of intramembranous bone and sutures upon in vivo cyclic tensile and compressive
Responses of intramembranous bone and sutures upon in vivo cyclic tensile and compressive
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DOI：
10.1016/j.bone.2007.05.014
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发表时间：
2008-02-01
期刊：
影响因子：
4.1
通讯作者：
Mao, Jeremy J.
中科院分区：
文献类型：
--
作者：
Peptan, Alexandra I.;Lopez, Aurora;Mao, Jeremy J.
颅顶和面部缝合线插入颅骨的矿化骨之间，并且可以类似于阑尾和颅底生长板发挥作用。然而，与由软骨细胞谱系组成的生长板不同，颅骨和面部缝合线除了血管源性细胞外，还具有异质细胞谱系，如间充质细胞、成纤维细胞和成骨细胞。尽管最近加大了努力，但膜内骨和缝合线对机械载荷的生物反应尚不清楚。本研究旨在调查短暂剂量的拉伸或压缩力是否会诱导膜内骨和缝合线的建模和生长反应。在体内生长的不同组兔子中，连续 12 天对上颌骨施加 1 N 和 8 Hz 的循环拉伸或压缩力，持续 20 分钟/天。计算机组织形态计量学分析显示，在张力或压缩载荷下，前上颌骨缝线（PMS）和鼻额缝线（NFS）的平均缝线宽度显着高于年龄和性别匹配的假手术对照组（P < 0.01）。拉伸或压缩负载的 PMS 和 NFS 的平均细胞密度显着高于假对照组（P < 0.01）。张力加载下成骨细胞平均占据骨缝骨表面显着高于假手术对照组（P < 0.05）。有趣的是，与假手术对照相比，拉伸载荷显着减少了平均破骨细胞表面（P < 0.05）。对于 NFS，与假手术对照相比，拉伸载荷显着增加了平均成骨细胞占据的骨缝骨表面（P < 0.05）。同样对于 NFS 缝合线，与假手术对照相比，压缩显着减少了平均缝合线破骨细胞表面（P < 0.05）。综上所述，目前的数据表明，拉伸或压缩的高频循环力会引起颅缝的建模和生长变化。由于颅顶和面部缝线的结构复杂性，拉力或压缩力可能以剪切应力的形式传递，并上调负责缝线生长的基因和基因产物。 (c) 2007 Elsevier Inc. 保留所有权利。
Cranial vault and facial sutures interpose between mineralized bones of the skull, and may function analogously to appendicular and cranial base growth plates. However, unlike growth plates that are composed of chondrocyte lineage, cranial and facial sutures possess heterogeneous cell lineages such as mesenchymal cells, fibroblasts, and osteoblasts, in addition to vascular-derived cells. Despite recently intensified effort, the biological responses of intramembranous bone and sutures to mechanical loading are not well understood. This study was designed to investigate whether brief doses of tensile or compressive forces induce modeling and growth responses of intramembranous bone and sutures. In different groups of growing rabbits in vivo, cyclic tensile or compressive forces at 1 N and 8 Hz were applied to the maxilla for 20 min/day over 12 consecutive days. Computerized histomorphometric analyses revealed that the average sutural widths of both the premaxillomaxillary suture (PMS) and nasofrontal suture (NFS) loaded in either tension or compression were significantly higher than age- and sex-matched sham controls (P < 0.01). The average cell densities of tension- or compression-loaded PMS and NFS were significantly higher than sham controls (P < 0.01). The average osteoblast occupied sutural bone surface loaded under tension was significantly higher than that of sham control (P < 0.05). Interestingly, tensile loading significantly reduced the average osteoclast surface, in comparison to sham control (P < 0.05). For the NFS, tensile loading significantly increased the average osteoblast occupied sutural bone surface, in comparison with that of sham control (P < 0.05). Also for the NFS suture, compression significantly reduced the average sutural osteoclast surface in comparison with sham control (P < 0.05). Taken together, the present data suggest that high-frequency cyclic forces in either tension or compression induce modeling and growth changes in cranial sutures. Due to the structural complexity of cranial vault and facial sutures, either tensile or compressive forces likely are transmitted as shear stresses and upregulate genes and gene products responsible for sutural growth. (c) 2007 Elsevier Inc. All rights reserved.