Expression of NDRG2 is down-regulated in high-risk adenomas and colorectal carcinoma.
Expression of NDRG2 is down-regulated in high-risk adenomas and colorectal carcinoma.
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DOI：
10.1186/1471-2407-7-192
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发表时间：
2007-10-12
期刊：
影响因子：
3.8
通讯作者：
Mitchelmore C
中科院分区：
文献类型：
--
作者：
Lorentzen A;Vogel LK;Lewinsky RH;Saebø M;Skjelbred CF;Godiksen S;Hoff G;Tveit KM;Lothe IM;Ikdahl T;Kure EH;Mitchelmore C
最近已经表明，NDRG 2 mRNA在几种人类癌症和癌细胞系中下调或检测不到。虽然NDRG 2的功能尚不清楚，但NDRG 2高表达与高级别胶质瘤的预后改善相关。本研究的目的是检测NDRG 2 mRNA在结肠癌中的表达。通过检查患有结直肠腺瘤和癌的个体以及健康个体的受影响和正常组织，我们的目的是确定在结肠癌发生过程中是否以及在哪个阶段发生NDRG 2下调。使用定量RT-PCR，我们已经确定了NDRG 2的mRNA水平在低风险（n = 15）和高风险腺瘤（n = 57），结直肠癌（n = 50）和相应的正常组织，以及对照组织从健康个体（n = 15）。NDRG 2水平相对于β-肌动蛋白标准化。与来自对照组的正常组织相比，结直肠癌中NDRG 2 mRNA水平较低（p < 0.001）。当比较来自同一个体的腺瘤/癌与邻近正常组织时，NDRG 2表达水平在高危腺瘤（p < 0.001）和结直肠癌（p < 0.001）中均显著降低。NDRG 2水平随Dukes分期的增加而降低（p < 0.05）。我们的研究结果表明，NDRG 2的表达下调，在大肠癌发生的晚期阶段。未来的研究需要解决NDRG 2下调是否是结直肠腺瘤进展为癌的原因或结果。
It has recently been shown that NDRG2 mRNA is down-regulated or undetectable in several human cancers and cancer cell-lines. Although the function of NDRG2 is unknown, high NDRG2 expression correlates with improved prognosis in high-grade gliomas. The aim of this study has been to examine NDRG2 mRNA expression in colon cancer. By examining affected and normal tissue from individuals with colorectal adenomas and carcinomas, as well as in healthy individuals, we aim to determine whether and at which stages NDRG2 down-regulation occurs during colonic carcinogenesis. Using quantitative RT-PCR, we have determined the mRNA levels for NDRG2 in low-risk (n = 15) and high-risk adenomas (n = 57), colorectal carcinomas (n = 50) and corresponding normal tissue, as well as control tissue from healthy individuals (n = 15). NDRG2 levels were normalised to β-actin. NDRG2 mRNA levels were lower in colorectal carcinomas compared to normal tissue from the control group (p < 0.001). When comparing adenomas/carcinomas with adjacent normal tissue from the same individual, NDRG2 expression levels were significantly reduced in both high-risk adenoma (p < 0.001) and in colorectal carcinoma (p < 0.001). There was a trend for NDRG2 levels to decrease with increasing Dukes' stage (p < 0.05). Our results demonstrate that expression of NDRG2 is down-regulated at a late stage during colorectal carcinogensis. Future studies are needed to address whether NDRG2 down-regulation is a cause or consequence of the progression of colorectal adenomas to carcinoma.
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