Comparative Morpho-Anatomical and HPTLC Profiling of Tinospora Species and Dietary Supplements
Comparative Morpho-Anatomical and HPTLC Profiling of Tinospora Species and Dietary Supplements
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DOI：
10.1055/a-1120-3711
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发表时间：
2020-05-01
期刊：
影响因子：
2.7
通讯作者：
Khan, Ikhlas A.
中科院分区：
文献类型：
--
作者：
Parveen, Abidah;Adams, John S.;Khan, Ikhlas A.
重叠的地理分布，传统使用的历史，物种鉴定的混乱，以及不同物种之间的形态相似性是一些考虑因素，需要进行有效的质量控制和更安全的植物产品的定性分析的重要性。本文对心叶青牛胆、皱叶青牛胆、中华青牛胆的叶、茎和班氏青牛胆的茎进行了详细的形态解剖。对所选青牛胆属物种的显微镜研究揭示了关键的诊断特征，可以帮助区分密切相关的青牛胆属物种，以及检测原材料中的任何掺假或替代。比较了经鉴定的植物材料以及声称含有青牛胆属的商业产品的HPTLC谱，以区分青牛胆属。crispa从其他密切相关的物种，并建立一个有效的方法来评估的身份和质量的产品使用合格的化学标记。植物样品和膳食补充剂的HPTLC色谱图与六个参考标记化合物进行了比较。分析表明，硼硼甙B和C可作为鉴别T. crispa，而蒂诺斯苷A是鉴定T.中国产品。
Overlapping geographical occurrence, history of traditional use, confusion in species identification, and morphological resemblances among various species are some considerations that necessitate the importance of qualitative analysis for efficient quality control and safer botanical products. This paper provides detailed morpho-anatomies of the leaves and stems of Tinospora cordifolia, Tinospora crispa , and Tinospora sinensis , and stems of Tinospora baenzigeri . Microscopy studies of the selected Tinospora species revealed key diagnostic features that can help distinguish the closely related species of Tinospora as well as to detect any adulteration or substitution in the raw materials. HPTLC profiles of the authenticated plant materials, as well as commercial products claiming to contain Tinospora , were compared to distinguish T. crispa from other closely related species and to establish an efficient method to assess the identity and quality of the products using qualified chemical markers. HPTLC chromatograms of both plant samples and dietary supplements were compared with six reference marker compounds. The analysis revealed that borapetoside B and C were useful to identify T. crispa while tinosineside A was found to be characteristic to authenticate the T. sinensis products.