Lectin-Based Biosensor Strategy for Electrochemical Assay of Glycan Expression on Living Cancer Cells
Lectin-Based Biosensor Strategy for Electrochemical Assay of Glycan Expression on Living Cancer Cells
复制标题
基于凝集素的生物传感器策略，用于电化学测定活癌细胞上聚糖的表达
DOI：
10.1021/ac102132p
复制
发表时间：
2010-11-15
影响因子：
7.4
通讯作者：
Zhang, Wen
中科院分区：
文献类型：
--
作者：
Zhang, Xinai;Teng, Yingqiao;Zhang, Wen
在这篇文章中，我们报告了一种新的基于凝集素的生物传感器的电化学检测癌症相关的糖基化的比较研究甘露糖和唾液酸的表达正常和癌细胞来自人肺，肝，前列腺。使用夹心形式，通过将基于凝集素的生物传感器与{凝集素-Au-Th}生物缀合物组合来实现高灵敏度和选择性，所述{凝集素-Au-Th}生物缀合物具有连接到金纳米颗粒（AuNP）的凝集素和硫杂环丁烷（11）标记物用于信号放大。所提出的策略表明，甘露糖在正常细胞和癌细胞中都表现出高表达水平，而唾液酸在癌细胞中比正常细胞更丰富。结果与荧光显微镜研究结果一致。两种聚糖表达的差异表明唾液酸可以作为早期癌症检测的潜在生物标志物。基于凝集素的生物传感器还成功地用于定量癌细胞和评估单个细胞表面上唾液酸的平均量，这可以提供关于聚糖在癌症进展中的功能的重要信息。总之，基于凝集素的电化学生物传感器提供了分析活细胞上聚糖表达的有效途径，并且可以极大地促进癌症早期过程的医学诊断和治疗。
In this article, we report a novel lectin-based biosensor for electrochemical assay of cancer-associated glycosylation by comparative study of mannose and sialic acid expression on normal and cancer cells derived from human lung, liver, and prostate. Using a sandwich format, high sensitivity and selectivity were achieved by combining the lectin-based biosensor with the {lectin-Au-Th} bioconjugates featuring lectin and thionine (11) labels linked to gold nanoparticles (AuNPs) for signal amplification. The proposed strategy demonstrated that mannose exhibited high expression levels in both normal and cancer cells, while sialic acid was more abundant in cancer cells as compared to normal ones. The results were in good agreement with those from fluorescent microscopy studies. The differences in the two glycan expression indicated that sialic acid could serve as a potential biomarker for early cancer detection. The lectin-based biosensor was also successfully used to quantify cancer cells and evaluate the average amount of sialic acid on single cell surface, which could supply significant information on glycan functions in cancer progression. Overall, the lectin-based electrochemical biosensor provides an effective pathway to analyze glycan expression on living cells and may greatly facilitate the medical diagnosis and treatment in early process of cancer.