Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-beta 1
Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-beta 1
复制标题
声动力疗法抑制 TGF-β1 诱导的大鼠心脏成纤维细胞的纤维形成
DOI：
10.1159/000452571
复制
发表时间：
2016
影响因子：
--
通讯作者：
Tian Ye
中科院分区：
文献类型：
--
作者：
Guo Yuanyuan;Dong Zengxiang;Shi Yuanqi;Wang Wei;Wang Lu;Sun Jing;Sun Xin;Tian Zhen;Yao Jianting;Li Zhitao;Cheng Jiali;Tian Ye
Background/AimsSonodynamic therapy (SDT) is a localized ultrasound-activated therapy for atherosclerosis when combined with a sonosensitizer, 5-aminolevulinic acid (ALA), but whether it can prevent cardiac fibrosis has not been studied. In the present study, we evaluated the effects SDT on fibrogenesis in rat cardiac fibroblasts.MethodsThe primary cardiac fibroblasts were isolated from rats, and induced to fibrogenesis with TGF-β1. With this in vitro model, we tested the preventive effects of SDT on fibrogenesis and further the underlying mechanism.ResultsTGF-β1 stimulation up-regulated α-SMA and COLI/III protein levels in cardiac fibroblasts, and enhanced the progression of cells from the G0/G1 phase to the S phase. SDT inhibited the TGF-β1 mediated cell proliferation and decreased the levels of α-SMA and COLI/III by activating AKT/GSK3β pathway and blocking TGF-β1/SMAD3 signaling.ConclusionOur studies demonstrate an antifibrotic effect of SDT in rat cardiac fibroblasts, suggesting that SDT may intervene cardiac fibrogenesis by regulating myocardial fibrotic remodeling.
