Single molecule based SNP detection using designed DNA carriers and solid-state nanopores
Single molecule based SNP detection using designed DNA carriers and solid-state nanopores
复制标题
DOI：
10.1039/c6cc08621g
复制
发表时间：
2017-01-07
影响因子：
4.9
通讯作者：
Keyser, Ulrich F.
中科院分区：
文献类型：
--
作者：
Kong, Jinglin;Zhu, Jinbo;Keyser, Ulrich F.
单核苷酸多态性（snp）作为分子标记在医学和诊断中发挥着至关重要的作用。我们展示了一种基于纳米孔的单分子SNP检测方法。设计的DNA载体用于区分仅含有一个碱基差异的DNA链，并遵循链位移动力学。
Single nucleotide polymorphisms (SNPs) play a crucial role as molecular markers in medical and diagnostic application. We demonstrate a nanopore based method for SNP detection at the single molecule level. Designed DNA carriers are used to distinguish DNA strands containing only one single base difference and follow strand displacement kinetics.