A METHOD TO QUANTIFY GLUCOSE-UTILIZATION INVIVO IN SKELETAL-MUSCLE AND WHITE ADIPOSE-TISSUE OF THE ANESTHETIZED RAT
A METHOD TO QUANTIFY GLUCOSE-UTILIZATION INVIVO IN SKELETAL-MUSCLE AND WHITE ADIPOSE-TISSUE OF THE ANESTHETIZED RAT
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DOI：
10.1042/bj2280103
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
GIRARD, J
中科院分区：
文献类型：
--
作者：
FERRE, P;LETURQUE, A;GIRARD, J
提出了一种定量方法，允许在麻醉大鼠的肌肉和白色脂肪组织体内确定葡萄糖代谢。注射了2-脱氧[3H]葡萄糖的示踪剂剂量。在麻醉大鼠和2-脱氧[3H]葡萄糖的浓度中被监测在动脉血液中。 30-80分钟后，对3个肌肉，比目鱼，伸肌长肌和表质chlel，表周围的白色脂肪组织和大脑进行采样，并分析其2-脱氧[3H]葡萄糖6-磷酸的含量。该含量可能与同一时期内的葡萄糖利用有关，因为已知2-脱氧[3H]葡萄糖的积分是已知的，并且与运输中葡萄糖相比，校正因子的校正因子对2-脱氧葡萄糖的模拟效应的校正因子校正了。从体外实验确定磷酸化步骤。然后，通过该技术在基底状态的吸收后大鼠组织中（0.1胰岛素/毫升等离子体的胰岛素/mL）或在尤利克斯流行 - 高血糖 - 高透明葡萄糖夹（8的胰岛素/血浆胰岛素/ML）和48的葡萄糖 - 血糖 - 血糖蛋白酶钳（48）和48 H-Starved大鼠。结果在定性和定量上与所研究组织的已知生理特征相对应。
A quantitative method allowing determination of glucose metabolism in vivo in muscles and white adipose tissue of the anesthetized rat is presented. A tracer dose of 2-deoxy[3H]glucose was injected i.v. in an anesthetized rat and the concentration of 2-deoxy[3H]glucose was monitored in arterial blood. After 30-80 min, 3 muscles, the soleus, the extensor digitorum longus and the epitrochlearis, periovarian white adipose tissue and brain were sampled and analyzed for their content of 2-deoxy[3H]glucose 6-phosphate. This content could be related to glucose utilization during the same time period, since the integral of the decrease of 2-deoxy[3H]glucose in arterial blood was known and correction factors for the analogue effect of 2-deoxyglucose compared with glucose in the transport and phosphorylation steps were determined from experiments in vitro. Glucose utilization was then measured by this technique in the tissues of post-absorptive rats in the basal state (0.1 munit of insulin/ml of plasma) or during euglycemic-hyperinsulinemic glucose clamp (8 munits of insulin/ml of plasma) and of 48 h-starved rats. Results corresponded qualitatively and quantitatively to the known physiological characteristics of the tissues studied.