Tissue-specific expression of TRP channel genes in the mouse and its variation in three different mouse strains.
Tissue-specific expression of TRP channel genes in the mouse and its variation in three different mouse strains.
复制标题
TRP通道基因在小鼠中的组织特异性表达及其在三种不同小鼠菌株中的变异。
DOI：
10.1186/1471-2164-7-159
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发表时间：
2006-06-20
期刊：
影响因子：
4.4
通讯作者：
Brinkmeier, Heinrich
中科院分区：
文献类型：
--
作者：
Kunert-Keil, Christiane;Bisping, Frederike;Kruger, Jana;Brinkmeier, Heinrich
本工作的目的是研究小鼠瞬时受体电位(Trp)通道的基因表达。一种标准化和定量的技术TaqMan RT-PCR的应用，应该可以提供关于TRP通道的模式和对小鼠组织和细胞类型的相对重要性的信息。为了用独立的方法验证数据集，我们通过原位杂交研究了一些转录本的出现。我们已经确定了22个Trp通道在小鼠中的mRNA表达，重点是神经和肌肉组织。这是首次用标准化和定量技术描述了四个相关的Group 1亚家族(TRPC、TRPV、TRPM和TRPA)的所有通道亚型的表达谱。转录丰度的比较表明，TRPM7和TRPC3在大多数组织中具有一致的优势。我们进一步观察到个体通道的特征模式和基因表达的差异，范围超过三个数量级。Trp通道mRNAs的总体水平在脑区最高，其次是肾、肺、生殖器官和肌肉。在脑内，以TRPM3和TRPM7为主，检测到19种其他亚型。在肺和肾脏中，TRPV4、TRPV5和TRPM7含量最高。TRPM7、TRPC3、TRPC6和TRPM3mRNAs在所有受试肌肉组织中均有特征性表达。用C57BL/10小鼠获得的大部分数据与Balb/c和NOD小鼠的结果一致。然而，TRPC3、C6、TRPM7、M3、TRPV2和V4的表达在三个受试品系之间存在显著差异。原位杂交显示转录产物在细胞水平上共表达，并广泛证实了RT-PCR获得的数据。Trp阳离子通道的TRPC、TRPV、TRPM和TRPA亚家族成员的转录本存在于广泛的小鼠组织中。几种通道异构体通常共存于特定的组织或细胞类型中。Trp通道的表达不像其他离子通道家族那样表现出典型的组织特异性个体成员的优势。小鼠品系Trp通道表达的特异性变化表明，遗传背景或生理要求对表达水平有很大影响。
The purpose of this work was to study the gene expression of transient receptor potential (TRP) channels in the mouse. The application of a standardized and quantitative technique, TaqMan RT-PCR, should give information about the pattern and relative importance of TRP channels for murine tissues and cell types. To verify data sets with an independent method, we studied the occurrence of some of the transcripts by in situ hybridization. We have characterized the mRNA expression of 22 TRP channels in the mouse with a focus on nerve and muscle tissues. This is the first study to describe the expression profiles of all channel isoforms of the four related Group 1 subfamilies (TRPC, TRPV, TRPM and TRPA) with a standardized and quantitative technique. Comparisons of transcript abundance showed a consistent dominance of TRPM7 and TRPC3 in most tissues. We further observed characteristic patterns and differences in gene expression of individual channels ranging over three orders of magnitude. The overall level of TRP channel mRNAs was highest in brain areas followed by kidney, lung, reproductive organs and muscle. In brain TRPM3 and TRPM7 dominated and 19 other isoforms were detected. In lung and kidney TRPV4, TRPV5 and TRPM7 were found in highest levels. TRPM7, TRPC3, TRPC6 and TRPM3 mRNAs were characteristically present in all tested muscle tissues. Most data obtained with the C57Bl/10 mouse strain were confirmed with Balb/c and NOD mice. However, TRPC3, C6, TRPM7, M3, TRPV2 and V4 expression showed marked differences in the three tested mouse strains. In situ hybridization revealed co-expression of transcripts on the cellular level and widely confirmed the data obtained with RT-PCR. Transcripts coding for members of the TRPC, TRPV, TRPM and TRPA subfamilies of TRP cation channels are present in a broad spectrum of murine tissues. Several channel isoforms often coexist in a specific tissue or cell type. TRP channel expression does not show typical tissue specific dominance of individual members as is known from other ion channel families. Mouse strain specific variations of TRP channel expression indicate that genetic background or physiological requirements considerably influence expression levels.