Determination of arsenic and lead in single hair strands by laser ablation inductively coupled plasma mass spectrometry.
Determination of arsenic and lead in single hair strands by laser ablation inductively coupled plasma mass spectrometry.
复制标题
激光烧蚀电感耦合等离子体质谱法测定单根头发中的砷和铅
DOI：
10.1038/s41598-017-03660-6
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发表时间：
2017-06-13
影响因子：
4.6
通讯作者：
Ma D
中科院分区：
文献类型：
--
作者：
Luo R;Su X;Xu W;Zhang S;Zhuo X;Ma D
本文建立了基质匹配的头发标准品和单点扫描LA-ICP-MS法同时测定人发中As和Pb的方法。这些结果随后可以用来推断元素何时进入人体。本研究分两部分进行。首先，开发并验证了一种方法的头发元素分析LA-ICP-MS。在LA-ICP-MS的校准策略，开发使用制备的基质匹配的实验室头发标准掺杂的分析物在一个定义的浓度。使用发束标准品能够通过绘制分析物离子（M+）强度（标准化为34 S+（M+/34 S+比值））作为酸性荧光素的ICP-MS测定浓度的函数来获得校准曲线。分析物As和Pb的校准曲线的线性相关系数（R2）通常分别在0.9970和0.9998之间。其次，使用开发的方法测量实际头发。在用三氧化二砷（As 2 O3）治疗的白血病患者的头发样品中观察到As沿着头发的空间分布。对药物进入体内的实际时间和估计时间进行了比较和讨论。
The purpose of this study was to develop matrix-matched hair standards and a LA-ICP-MS technique for determination of the As and Pb in a single human hair using single spot scan mode. These results could subsequently be used to infer when the element entered the body. This study was conducted in two parts. First, a method was developed and validated for the elemental analysis of hair by LA-ICP-MS. A calibration strategy in LA-ICP-MS was developed using prepared matrix-matched laboratory hair standards doped with analytes of interest at a defined concentration. The use of hair strand standards enables calibration curves to be obtained by plotting the analyte ion (M+) intensity normalized to34S+(the ratio M+/34S+) as a function of the concentration determined by ICP-MS of the acidic digests. The linear correlation coefficients (R2) of the calibration curves for the analytes As and Pb were typically between 0.9970 and 0.9998, respectively. Second, an actual hair was measured using the developed method. The spatial distribution of As along the hair was observed in a hair sample from a leukaemia patient treated with arsenic trioxide (As2O3). The actual and estimated times over which the drug entered the body were compared and discussed.