FREQUENT OCCURRENCE OF HIV-INHIBITORY SULFATED POLYSACCHARIDES IN MARINE-INVERTEBRATES
FREQUENT OCCURRENCE OF HIV-INHIBITORY SULFATED POLYSACCHARIDES IN MARINE-INVERTEBRATES
复制标题
DOI：
10.1177/095632029300400306
复制
发表时间：
1993-01-01
影响因子：
--
通讯作者：
BOYD, MR
中科院分区：
文献类型：
--
作者：
BEUTLER, JA;MCKEE, TC;BOYD, MR
许多海洋无脊椎动物的水提取物在国家癌症研究所的抗HIV细胞病变的初步筛选中表现出一些活性。使用各种技术，包括凝胶渗透，尺寸排阻和离子交换色谱法，甲苯胺蓝异染性，C-13-NMR光谱和燃烧分析，我们已经确定，这种活动主要是由于硫酸多糖。由于这类化合物在这些生物体中广泛存在，我们寻求一种快速去复制硫酸多糖的方法。关键是选择去复制的方法允许阴离子多糖与其他AIDS-抗病毒化学型的区分。在评估了各种方法后，我们发现最有效的策略似乎是从粗水提取物的乙醇水溶液中沉淀多糖级分。
Aqueous extracts of many marine invertebrates have exhibited some activity in the National Cancer Institute's primary screen for anti-HIV cytopathicity. Using a variety of techniques, including gel permeation, size exclusion and ion exchange chromatography, toluidine blue metachromicity, C-13-NMR spectroscopy and combustion analyses, we have determined that this activity is largely due to sulphated polysaccharides. Because of the wide occurrence of this class of compounds in these organisms we sought a method for the rapid dereplication of sulphated polysaccharides. It was critical that the method selected for dereplication allow differentiation of anionic polysaccharides from other AIDS-antiviral chemotypes. After evaluating a variety of methods, we found that the most efficient strategy appeared to be precipitation of the polysaccharide fraction from aqueous ethanolic solutions of the crude aqueous extracts.