Cathepsin D is secreted from M-BE cells: Its potential role as a biomarker of lung cancer
Cathepsin D is secreted from M-BE cells: Its potential role as a biomarker of lung cancer
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DOI：
10.1021/pr060422t
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发表时间：
2007-03-01
影响因子：
4.4
通讯作者：
Xu, Ningzhi
中科院分区：
文献类型：
--
作者：
Lou, Xiaomin;Xiao, Ting;Xu, Ningzhi
肺癌的早期诊断是降低恶性肿瘤死亡率的有效途径。为探索早期肺癌血清标志物，采用SV 40 T转化的人支气管上皮细胞系M-BE，在高传代时具有早期成瘤表型特征，在条件培养基中培养，收集其分泌蛋白。提取不同代次的M-BE细胞分泌的蛋白质，用双向电泳（2-DE）进行分离。采用MALDI-TOF/TOF质谱技术对蛋白质组进行双向电泳鉴定。共鉴定出47个蛋白，其中23个上调，24个下调。在这些蛋白质中，组织蛋白酶D是典型的分泌蛋白，其在培养基中或在传代过程中的细胞中表现出增加的丰度。此外，蛋白质组学的结论在肺癌患者的临床样本中得到了验证。用双抗体夹心ELISA法检测104例肺鳞癌患者血浆组织蛋白酶D浓度，与36例正常人比较，差异有显著性（P <0.05）。
The early diagnosis of lung cancer is an effective approach to reduce the mortality caused by malignancy. To explore serum biomarkers of lung cancer at early stage, M-BE, a SV40T-transformed human bronchial epithelial cell line with the phenotypic features of early tumorigenesis at high passage, was cultured in the conditioned media to collect its secretory proteins. The proteins secreted from different passage M-BE cells were extracted and then separated by two-dimensional electrophoresis (2-DE). MALDI-TOF/TOF mass spectrometry was adopted to identify the passage-dependent 2-DE spots. Totally, 47 proteins were identified, including 23 that were up-regulated and 24 that were down-regulated. Of these proteins, cathepsin D was a typical secretory protein that exhibited the increased abundance either in culture media or in cells during passaging. Furthermore, the proteomic conclusions were validated in the clinical samples of lung cancer patients. When sandwich ELISA was used, the concentrations of cathepsin D in plasma showed significant differences between lung squamous cell carcinomas (SCC, 104 cases) and normal donors (36 cases, p