csi2p modulates microtubule dynamics and organizes the bipolar spindle for chromosome segregation.
csi2p modulates microtubule dynamics and organizes the bipolar spindle for chromosome segregation.
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DOI：
10.1091/mbc.e14-09-1370
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发表时间：
2014-12-01
影响因子：
3.3
通讯作者：
Tran PT
中科院分区：
文献类型：
--
作者：
Costa J;Fu C;Khare VM;Tran PT
报道了一个新基因csi2+(染色体分离受损2)。它定位于纺锤体极体，它的缺失导致瞬时单极纺锤体和随后的染色体滞后。有人认为，csi2p调节有丝分裂微管的长度，其中的缺陷可能导致动粒-微管附着问题。适当的染色体分离对于适当的遗传是至关重要的。染色体分离的缺陷会导致非整倍体，这是癌细胞的一个特征。真核细胞的染色体分离是由两极纺锤体完成的。其他机构，如主轴组件检查点和着丝粒定位，进一步有助于确保完全分离的保真度。在这里，我们介绍了裂解酵母csi2+。Csi2p定位于纺锤体极，在那里它调节有丝分裂微管的动力学，双极纺锤体的形成，以及随后的染色体分离。CSI2缺失(CSI2Δ)导致有丝分裂微管异常长，瞬时单极纺锤体发生率高，随后染色体分离缺陷率高。由于CSI2Δ具有多种表型，因此它能够估计不同机制对整个染色体分离过程的相对贡献。着丝粒定位、微管动力学和两极纺锤体的形成都有助于染色体分离。然而，分裂酵母染色体分离缺陷的主要决定因素可能是微管动态缺陷。
A novel gene, csi2+ (chromosome segregation impaired 2), is reported. It localizes to the spindle pole body, and its deletion leads to a transient monopolar spindle and subsequent chromosome lagging. It is proposed that csi2p regulates mitotic microtubule length, defects in which may cause kinetochore–microtubule attachment problems. Proper chromosome segregation is of paramount importance for proper genetic inheritance. Defects in chromosome segregation can lead to aneuploidy, which is a hallmark of cancer cells. Eukaryotic chromosome segregation is accomplished by the bipolar spindle. Additional mechanisms, such as the spindle assembly checkpoint and centromere positioning, further help to ensure complete segregation fidelity. Here we present the fission yeast csi2+. csi2p localizes to the spindle poles, where it regulates mitotic microtubule dynamics, bipolar spindle formation, and subsequent chromosome segregation. csi2 deletion (csi2Δ) results in abnormally long mitotic microtubules, high rate of transient monopolar spindles, and subsequent high rate of chromosome segregation defects. Because csi2Δ has multiple phenotypes, it enables estimates of the relative contribution of the different mechanisms to the overall chromosome segregation process. Centromere positioning, microtubule dynamics, and bipolar spindle formation can all contribute to chromosome segregation. However, the major determinant of chromosome segregation defects in fission yeast may be microtubule dynamic defects.