Pt-Ru bimetallic nanoclusters with super peroxidase-like activity for ultra-sensitive lateral flow immunoassay
Pt-Ru bimetallic nanoclusters with super peroxidase-like activity for ultra-sensitive lateral flow immunoassay
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具有超过氧化物酶样活性的 Pt-Ru 双金属纳米团簇用于超灵敏侧流免疫分析
DOI：
10.1016/j.cej.2023.141324
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发表时间：
2023-02
影响因子：
15.1
通讯作者：
Zhijun Huang
中科院分区：
文献类型：
--
作者：
Xiaoting Li;Cheng Qian;Yonghui Tian;Naizhi Yao;Yixiang Duan;Zhijun Huang
本文采用一锅水热法制备了Pt-Ru纳米团簇。所制备的Pt-Ru纳米颗粒具有极高的过氧化物酶样活性，比活性为115.36 U/mg，远远上级Pt纳米颗粒和许多报道的单原子纳米酶。这可以归因于各种微结构有效的催化和独特的电子转移特性，导致活性羟基自由基（自由基dotOH）的激活，如密度泛函理论所揭示的。以Pt-Ru NCs作为辣根过氧化物酶的替代物，与抗体交联后作为催化标记，建立了检测D-二聚体的比色增强侧流免疫分析法（LFIA）。因此，仅需20 s的额外显色，视觉灵敏度可提高约100倍，达到1 ng/mL，同时保持良好的特异性和抗干扰性。总体而言，Pt-Ru纳米粒子的优异性能表明其在比色检测方面具有很大的潜力，值得进一步推广。
In this work, Pt-Ru bimetallic nanoclusters (Pt-Ru NCs) were prepared by a simple one-pot hydrothermal method. The as-prepared Pt-Ru NCs has extremely high peroxidase-like activity, showing a specific activity of 115.36 U/mg far superior to Pt NCs and many reported single-atom nanozymes. This can be attributed to the various microstructures effective for catalysis and unique electron transfer characteristics leading to active hydroxyl radicals (radical dotOH) activation as revealed by density functional theory. By using the Pt-Ru NCs as a substitute for natural horseradish peroxidaseand when cross-linking with antibody as a catalytic label, the colorimetric enhanced lateral flow immunoassay (LFIA) for D-dimer detection was established. As a result, with only 20 s of additional coloration, the visual sensitivity can be improved by about 100 times to 1 ng/mL, while maintaining good specificity and anti-interference. In general, the excellent performance indicates that the Pt-Ru NCs have great potential in colorimetric detection, which is worthy of further promotion.
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影响因子：
--
作者：
Xiaoqing Huang
通讯作者：
Xiaoqing Huang
NADPH 引导的碘响应纳米酶的合成：纳米簇生长和过氧化物酶样活性的协同效应
影响因子：
4.5
作者：
Zheng Shanshan;Zhang Qianqian;Yin Danyang;Gu Hongzhi;Zhang Jinli;Li Wei;Fu Yan
通讯作者：
Fu Yan
DOI：
10.1021/la047555i
发表时间：
2005-01
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Peter N. Njoki;Jin Luo;Lingyan Wang;M. Maye;Huzaifa Quaizar;C. Zhong
通讯作者：
Peter N. Njoki;Jin Luo;Lingyan Wang;M. Maye;Huzaifa Quaizar;C. Zhong
影响因子：
6.2
作者：
Smally Aj
通讯作者：
Smally Aj
影响因子：
--
作者：
Yanqing Wang;Yuemeng Li;Liping Ding;Yanfeng Tang;J. Ding
通讯作者：
Yanqing Wang;Yuemeng Li;Liping Ding;Yanfeng Tang;J. Ding