Tang-Luo-Ning Improves Mitochondrial Antioxidase Activity in Dorsal Root Ganglia of Diabetic Rats: A Proteomics Study.
Tang-Luo-Ning Improves Mitochondrial Antioxidase Activity in Dorsal Root Ganglia of Diabetic Rats: A Proteomics Study.
复制标题
唐洛宁提高糖尿病大鼠背根神经节线粒体抗氧化酶活性：蛋白质组学研究。
DOI：
10.1155/2017/8176089
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发表时间：
2017
影响因子：
--
通讯作者：
Yi W
中科院分区：
文献类型：
--
作者：
Zhang T;Gao Y;Gong Y;Zhou H;Xie P;Guan S;Yi W
糖络宁是治疗糖尿病周围神经病变的传统中药。在这项研究中，我们研究了线粒体蛋白质谱在糖尿病大鼠模型，并探讨了潜在的保护作用TLN。采用链脲佐菌素（STZ）诱导糖尿病大鼠模型，分为模型组、α-硫辛酸（ALA）组和TLN组。从背根神经节中分离线粒体蛋白，并使用蛋白质组学分析来定量差异表达的蛋白质。糖络宁可减轻STZ诱导的糖尿病症状和血糖水平，包括对冷热刺激的反应时间和神经传导速度。与正常对照组比较，TLN组差异表达蛋白388个，ALA组差异表达蛋白445个，模型组差异表达蛋白451个。与模型组比较，TLN组差异蛋白275个，ALA组差异蛋白251个。与模型组相比，TLN组线粒体复合物III明显减少，谷胱甘肽过氧化物酶和过氧化物酶明显增加。与ALA组相比，TLN组线粒体复合物III含量增加，复合物IV含量减少。总之，TLN应该具有很强的抗氧化活性，这似乎是通过调节呼吸复合物和抗氧化酶来调节的。
Tang-luo-ning (TLN) is a traditional Chinese herbal recipe for treating diabetic peripheral neuropathy (DPN). In this study, we investigated mitochondrial protein profiles in a diabetic rat model and explored the potential protective effect of TLN. Diabetic rats were established by injection of streptozocin (STZ) and divided into model, alpha lipoic acid (ALA), and TLN groups. Mitochondrial proteins were isolated from dorsal root ganglia and proteomic analysis was used to quantify the differentially expressed proteins. Tang-luo-ning mitigated STZ-induced diabetic symptoms and blood glucose level, including response time to cold or hot stimulation and nerve conductive velocity. As compared to the normal, there were 388 differentially expressed proteins in the TLN group, 445 in ALA group, and 451 in model group. As compared to the model group, there were 275 differential proteins in TLN group and 251 in ALA group. As compared to model group, mitochondrial complex III was significantly decreased, while glutathione peroxidase and peroxidase were increased in TLN group. When compared with ALA group, the mitochondrial complex III was increased, and mitochondrial complex IV was decreased in TLN group. Together, TLN should have a strong antioxidative activity, which appears to be modulated through regulation of respiratory complexes and antioxidases.