High shikimate production from quinate with two enzymatic systems of acetic acid bacteria
High shikimate production from quinate with two enzymatic systems of acetic acid bacteria
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DOI：
10.1271/bbb.60259
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发表时间：
2006-10-01
影响因子：
1.6
通讯作者：
Matsushita, Kazunobu
中科院分区：
文献类型：
--
作者：
Adachi, Osao;Ano, Yoshitaka;Matsushita, Kazunobu
在醋酸菌的细胞质膜中，通过两个连续的酶反应，即喹蛋白奎尼酸脱氢酶（QDH）和3 - 脱氢奎尼酸脱水酶，奎尼酸经3 - 脱氢奎尼酸生成3 - 脱氢莽草酸，产率为57 - 77%。然后，3 - 脱氢莽草酸被来自同一生物体的依赖NADP的莽草酸脱氢酶（SKDH）还原为莽草酸（SKA）。当在过量D - 葡萄糖存在下，SKDH与依赖NADP的D - 葡萄糖脱氢酶（GDH）偶联作为NADPH再生系统时，SKDH会持续产生SKA，直至反应混合物中最初添加的3 - 脱氢莽草酸完全转化为SKA。基于所提供的数据，提出了一种高产SKA的策略。
3-Dehydroshikimate was formed with a yield of 57-77% from quinate via 3-dehydroquinate by two successive enzyme reactions, quinoprotein quinate dehydrogenase (QDH) and 3-dehydroquinate dehydratase, in the cytoplasmic membranes of acetic acid bacteria. 3-Dehydroshikimate was then reduced to shikimate (SKA) with NADP-dependent SKA dehydrogenase (SKDH) from the same organism. When SKDH was coupled with NADP-dependent D-glucose dehydrogenase (GDH) in the presence of excess D-glucose as an NADPH regenerating system, SKDH continued to produce SKA until 3-dehydroshikimate added initially in the reaction mixture was completely converted to SKA. Based on the data presented, a strategy for high SKA production was proposed.