Production of Hexaploid Wheats with Waxy Endosperm Character
Production of Hexaploid Wheats with Waxy Endosperm Character
复制标题
具有蜡质胚乳特性的六倍体小麦的生产
DOI：
10.1094/cchem.1997.74.1.72
复制
发表时间：
1997
期刊：
影响因子：
2.4
通讯作者：
I. Yamaguchi
中科院分区：
文献类型：
--
作者：
C. Kiribuchi‐Otobe;T. Nagamine;T. Yanagisawa;M. Ohnishi;I. Yamaguchi
摘要 在我们的小麦育种计划中，将 Tanikei A6099 的低直链淀粉特性引入优良品系，意外地从 Saikai 168 × Tanikei A6099 的 F1 杂种的 249 个双单倍体系中获得了 5 个蜡质品系。所有蜡质线直链淀粉含量<1%，蓝色值<0.1。提取淀粉颗粒结合蛋白并进行改性十二烷基硫酸钠聚丙烯酰胺凝胶电泳。蜡质线缺乏 Wx 蛋白。淀粉糊粘度测量给出了糯小麦淀粉的糊化特性，其与非糯小麦的糊化特性有很大不同，但与糯玉米的糊化特性相似。然而，糯小麦的峰值粘度远高于糯玉米。
ABSTRACT In our wheat breeding program to introduce the low amylose character of Tanikei A6099 to elite lines, five waxy lines were unexpectedly obtained from 249 doubled haploid lines of the F1 hybrid of Saikai 168 × Tanikei A6099. The amylose content of all the waxy lines was <1% and the blue value was <0.1. Starch granule-bound proteins were extracted and subjected to modified sodium dodecyl sulfate polyacrylamide gel electrophoresis. The waxy lines lacked the Wx protein. Starch paste viscosity measurements gave pasting profiles of waxy wheat starch that were quite different from those of nonwaxy wheats but similar to those of waxy maize. However, the peak viscosity of waxy wheats was much higher than that of the waxy maize.