AEG-1 promotes angiogenesis and may be a novel treatment target for tongue squamous cell carcinoma
AEG-1 promotes angiogenesis and may be a novel treatment target for tongue squamous cell carcinoma
复制标题
DOI：
10.1111/odi.13300
复制
发表时间：
2020-07-01
期刊：
影响因子：
3.8
通讯作者：
Feng，Chong-Jin
中科院分区：
文献类型：
--
作者：
Chen，Shan;Chen，Lei-Hui;Feng，Chong-Jin
本研究探讨了星形胶质细胞升高基因- 1 （AEG - 1）在舌鳞癌（TSCC）细胞系血管生成中的潜在功能。方法采用鸡绒毛膜-尿囊膜（CAM）实验模型，对AEG - 1表达水平不同的TSCC细胞株进行不同程度的血管生成检测。接下来，我们将AEG - 1表达水平不同的TSCC细胞系移植到裸鼠身上，并通过免疫组织化学方法评估血管生成相关因子，即血管内皮生长受体因子2 （VEGFR - 2）和微血管密度（MVD）的表达。ELISA法检测血管内皮生长因子（VEGF）含量。结果AEG‐1‐下调um1细胞系血管数量显著减少（p< 0.05）， AEG‐1‐上调um2细胞系血管数量显著增加（p< 0.05）。此外，AEG‐1表达水平上调与更高的肿瘤血管生成有关，这反映在VEGF （p< 0.01）、VEGFR‐2 （p< 0.05）和MVD计数（p< 0.01）表达水平的增加上。结论AEG‐1可促进TSCC中肿瘤血管生成，通过抑制AEG‐1的表达抑制肿瘤血管生成可能是治疗TSCC的一种新的潜在途径。
BackgroundThe study explored the potential function of astrocyte elevated gene‐1 (AEG‐1) on angiogenesis in tongue squamous cell carcinoma (TSCC) in TSCC cell lines.MethodsThe different degrees of angiogenesis were detected in TSCC cell lines expressing different levels of AEG‐1 by chick chorioallantoic membrane (CAM) experimental model. Next, we established xenografts of different TSCC cell lines with different expression levels of AEG‐1 in nude mice and conducted immunohistochemistry to evaluate the expression of the angiogenesis‐associated factor, that is, vascular endothelial growth receptor factor 2 (VEGFR‐2) and microvessel density (MVD). Vascular endothelial growth factor (VEGF) was detected by ELISA.ResultsCAM assay showed that the number of vessels was significantly reduced in AEG‐1‐down um1 cell line (p< .05), whereas the number was significantly increased in AEG‐1‐over um2 cell line (p< .05). Moreover, up‐regulated AEG‐1 expression level was associated with higher tumor angiogenesis, which was reflected by augmented expression levels of VEGF (p< .01), VEGFR‐2 (p< .05), and MVD counting (p <.01).ConclusionsThis study demonstrated that AEG‐1 can promote tumor angiogenesis in TSCC and inhibition of tumor angiogenesis by repressing the expression of AEG‐1 may be a novel potential treatment approach for TSCC.