Ecologic genomics of DNA: Upstream bending in prokaryotic promoters
Ecologic genomics of DNA: Upstream bending in prokaryotic promoters
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DOI：
10.1101/gr.10.8.1185
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发表时间：
2000-08-01
期刊：
影响因子：
7
通讯作者：
Nevo, E
中科院分区：
文献类型：
--
作者：
Bolshoy, A;Nevo, E
在我们分析了预测的固有曲率沿所有可用完整原核基因组的分布后，将基因组分为两组。第一组所有原核生物的曲率分布表明，相当一部分启动子具有内在的DNA曲率，位于启动子区域的内部或上游。在第二类原核生物中，我们没有发现这种特殊的DNA曲率分布。值得注意的是，第一组的所有细菌都是中嗜性的，而第二组的许多原核生物是超嗜热性的。我们假设，与高温原核生物相比，DNA曲率在中温生物的基因调控中发挥着生物学作用，即DNA曲率可能具有由温度选择决定的功能适应意义。
After our analysis of the distribution of predicted intrinsic curvature along all available complete prokaryotic genomes, the genomes were divided into two groups. Curvature distribution in all prokaryotes of the first group indicated a substantial fraction of promoters characterized by intrinsic DNA curvature located within or upstream of the promoter region. We did not find this peculiar DNA curvature distribution in prokaryotes in the second group. Remarkably, all bacteria of the first group were mesophilic, whereas many prokaryotes of the second group were hyperthermophilic. We hypothesize that DNA curvature plays a biologic role in gene regulation in mesophilic as opposed to hyperthermophilic prokaryotes, i.e., DNA curvature presumably has a functional adaptive significance determined by temperature selection.