BADGE, BeadsArray for the Detection of Gene Expression, a high-throughput diagnostic bioassay
BADGE, BeadsArray for the Detection of Gene Expression, a high-throughput diagnostic bioassay
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DOI：
10.1101/gr.190901
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发表时间：
2001-11-01
期刊：
影响因子：
7
通讯作者：
Wang, X
中科院分区：
文献类型：
--
作者：
Yang, L;Tran, DK;Wang, X
目前有几种方法可用于基因表达分析。但是，它们中很少有适合于在数千个样本中快速、低成本地检测中等数量的基因。对于用于诊断和筛选的这种方法存在巨大需求。为了解决这一需求，我们已经开发了使用微球和Luminex制造的流体仪器进行基因表达分析的所有测定。该检测试剂盒被命名为基因表达检测微球阵列（BADGE）。BADGE在单个反应中可检测多达100个基因，杂交仅需1小时，读取检测过程中样本中所有100个基因的结果不到20秒。对于在五个独立的重复实验中检测到的基因，标准偏差<平均值的35%。我们已经监测多个发病相关的基因，同时在化学处理和控制拟南芥样品采用BADGE检测。将这些数据与从已建立的技术Affyssin GeneChip获得的数据进行比较。表达谱的变化非常相似。我们的研究表明，BADGE检测能够以负担得起的成本和快速的速度分析多个基因的表达。
Several methods are presently available for gene expression analysis. However, few of them are Suitable for detection of moderate numbers of genes in thousands of samples with high speed and low cost. There is great demand for such a method for use in diagnostics and screening. To address this need, we have developed all assay for gene expression analysis using microspheres and a fluidic instrument made by Luminex. The assay is named Beads Array for the Detection of Gene Expression (BADGE). BADGE call monitor up to 100 genes in a single reaction, and it takes only 1 h to hybridize and < 20 sec to read the results of all 100 genes in a sample for the detection process. For the genes detected in five independent replicate experiments, the standard deviation was < 35% of the mean. We have monitored multiple pathogenesis-related genes simultaneously in chemical-treated and control Arabidopsis samples employing the BADGE assay. The data were compared with those obtained from an established technology, Affymetrix GeneChip. The changes ill expression profiles were very similar. Our study showed that the BADGE assay was capable of profiling expression of multiple genes at affordable cost and rapid speed.