Evidence for the rapid phytotoxicity and environmental stress evaluation using the PAM fluorometric method: Importance and future application
Evidence for the rapid phytotoxicity and environmental stress evaluation using the PAM fluorometric method: Importance and future application
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DOI：
10.1023/a:1008955819527
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发表时间：
1999-12-01
期刊：
影响因子：
2.7
通讯作者：
Popovic, R
中科院分区：
文献类型：
--
作者：
Juneau, P;Popovic, R
调制荧光分析法为解释有关植物生理状态的光合参数开辟了新的可能性。通过将植物暴露于连续光化光和饱和光脉冲，可以从考茨基荧光瞬态计算四个重要值：Phi（M），作为最大光系统II（PSII）光化学的量度的最大量子产率; Phi '（M），作为电子传递平衡状态下的真实的PSII光化学的稳态量子产率; Q（P），光化学猝灭，作为通过光合电子传递的光能耗散的量度;和Q（N），非光化学猝灭，作为通过非光化学过程吸收的光能耗散的量度。这些PAM荧光参数的变化与植物的生理状态有关，因此它们可以作为不同环境胁迫效应的可靠指标。然而，这些参数从未被视为生态系统毒理学监测的有用标准工具。高灵敏度的调制荧光计是当今可用的，其允许在实验室或现场测量荧光参数。在这份报告中，我们评估使用这些参数在评估汞毒性羊角月牙藻和温度胁迫对番茄植株的快速环境影响。在解释我们的研究结果，我们建议在未来的生物测定技术先进的工具，这些参数的效用。
Modulated fluorometry has opened new possibilities for the interpretation of photosynthetic parameters concerning the physiological state of plants. By exposing plant to continuous actinic light and to pulses of saturating light, it is possible to calculate from the Kautsky fluorescence transient four important values: Phi(M), the maximum quantum yield as a measure of the maximum photosystem II (PSII) photochemistry; Phi'(M), the steady state quantum yield as a real PSII photochemistry at the equilibrium state of electron transport; Q(P), the photochemical quenching as a measure of light-energy dissipation via photosynthetic electron transport; and Q(N), the non-photochemical quenching as a measure of dissipation of the absorbed light energy via non-photochemical processes. The change of these PAM-fluorescence parameters is related to the physiological state of the plant and therefore they can be used as reliable indicators of different environmental stress effects. However, these parameters have never been seen as useful standard tools for toxicological monitoring of ecosystems. Highly sensitive modulated fluorometers are today available which allow to measure the fluorescence parameters either in the lab or in the field. In this report we evaluate the use of these parameters in assessing the rapid environmental impact of mercury toxicity on Selenastrum capricornutum and of temperature stress on tomato plants. In interpreting our results, we suggest the utility of some of these parameters as technologically advanced tools in future bioassays.