Effect of antiestrogens on EGF-mediated movement of human breast cancer cells.
Effect of antiestrogens on EGF-mediated movement of human breast cancer cells.
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抗雌激素对 EGF 介导的人乳腺癌细胞运动的影响。
DOI：
10.1159/000097867
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发表时间：
2007
期刊：
影响因子：
3.1
通讯作者：
Pento,JThomas
中科院分区：
文献类型：
--
作者：
Tong,GinaM;Rajah,TalithaT;Zang,Xiao-Ping;Bahr,StevenJ;Pento,JThomas
在之前的一项研究中，我们比较了几种生长因子对培养的乳腺癌细胞的影响，观察到表皮生长因子（EGF）增强了雌激素受体阳性乳腺癌细胞的侵袭性。本研究的目的是确定三种独特的抗雌激素对 EGF 介导的人类乳腺癌细胞运动的影响。使用延时视频显微镜 (TLVM) 测量 MCF-7 乳腺癌细胞的移动速率。 MCF-7 细胞用 10-6mol/l 的抗雌激素（他莫昔芬、ICI-182-780 (ICI) 或 1,1-二氯-顺-2,3-二芳基环丙烷 (AII)）预处理 4 天，然后在 TLVM 之前立即用 EGF (10-10mol/l) 处理。 EGF 在给药后 30-90 分钟增强了 MCF-7 细胞的运动能力。然而，EGF 介导的 MCF-7 细胞运动受到抗雌激素预处理的抑制，TAM 和 ICI 完全抑制 EGF 诱导的运动。总之，本研究表明 EGF 增强了 MCF-7 乳腺癌细胞的运动速率，而抗雌激素预处理可抑制 EGF 介导的运动。
In a previous study we compared the influence of several growth factors on breast cancer cells in culture and observed that epidermal growth factor (EGF) enhanced the invasiveness of estrogen receptor-positive breast cancer cells. The objective of the present study was to determine the influence of three unique antiestrogens on EGF-mediated movement of human breast cancer cells. The rate of movement of MCF-7 breast cancer cells was measured using time-lapse videomicroscopy (TLVM). The MCF-7 cells were pretreated with antiestrogen (either tamoxifen, ICI-182-780 (ICI) or 1,1-dichloro-cis-2,3-diarylcyclopropane (AII)) at 10–6mol/l for 4 days, and then treated with EGF (10–10mol/l) immediately prior to TLVM. EGF enhanced the motility of the MCF-7 cells at 30–90 min post-administration. However, EGF-mediated motility of the MCF-7 cells was inhibited by antiestrogen pretreatment, with TAM and ICI producing complete inhibition of EGF-induced motility. In conclusion, this study demonstrates that EGF enhances the rate of movement of MCF-7 breast cancer cells and that antiestrogen pretreatment inhibits EGF-mediated motility.