Derivatization-based sample-multiplexing for enhancing throughput in liquid chromatography/tandem mass spectrometry quantification of metabolites: an overview
Derivatization-based sample-multiplexing for enhancing throughput in liquid chromatography/tandem mass spectrometry quantification of metabolites: an overview
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DOI：
10.1016/j.chroma.2020.461679
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发表时间：
2020-12-20
影响因子：
4.1
通讯作者：
Ogawa, Shoujiro
中科院分区：
文献类型：
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作者：
Higashi, Tatsuya;Ogawa, Shoujiro
各种样品（包括体液、组织、细胞和食物）中代谢物的定量有助于我们了解它们在体内的生物活性和作用、许多疾病的诊断、药物和生物标志物的发现以及人类健康的许多方面。液相色谱（LC）/串联质谱（MS/MS）因其高特异性和灵敏度以及对各种化合物的广泛覆盖而成为代谢物定量的最强大和最可靠的方法。由于衍生代谢物的理想LC行为和增强的MS/MS检测能力，衍生化通常使LC/MS/MS的定量能力更强。另一方面，基于LC/MS/MS的定量在其分析通量方面有改进的空间。衍生化也是克服这一缺点的一种有前途的方法;通过使用多种精心设计的试剂对多个样品进行衍生化来实现在同一LC/MS/MS进样中对样品的多重化，可以提高通量。基于此背景，本文综述了基于衍生化的样品多路复用策略，特别是衍生化试剂的特点和应用，用于代谢物的LC/MS/MS定量分析。该策略已被用于各种代谢物的相对和绝对定量，并扩大了代谢物的覆盖范围。(C)2020 Elsevier B. V.保留所有权利。
The quantification of metabolites in various samples, including body fluids, tissues, cells, and foodstuffs, contributes to our understanding of their biological activities and roles in the body, diagnosis for many diseases, drug and biomarker discovery, and many aspects of human health. Liquid chromatography (LC)/tandem mass spectrometry (MS/MS) is the most powerful and reliable methodology for the quantification of metabolites due to its high specificity and sensitivity, and broad coverage of various compounds. Derivatization often makes the quantification power of LC/MS/MS stronger due to the desirable LC behavior and enhanced MS/MS detectability of the derivatized metabolites. On the other hand, LC/MS/MS-based quantification has room for improvement regarding its analysis throughput. Derivatization is also a promising approach to overcome this drawback; the multiplexing of samples in the same LC/MS/MS injection, which is achieved by derivatization of multiple samples with multiple well-designed reagents, can enhance the throughput. Based on this background, this article reviews the derivatization-based sample-multiplexing strategy, especially the characteristics and applications of the derivatization reagents, for the LC/MS/MS quantification of metabolites. This strategy has been used for the relative and absolute quantification of a variety of metabolites, and expansion of the coverage of metabolites. (C) 2020 Elsevier B.V. All rights reserved.