Biodegradation rates of 2-methylisoborneol (MIB) and geosmin through sand filters and in bioreactors
Biodegradation rates of 2-methylisoborneol (MIB) and geosmin through sand filters and in bioreactors
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DOI：
10.1016/j.chemosphere.2006.08.016
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发表时间：
2007-02-01
期刊：
影响因子：
8.8
通讯作者：
Newcombe, Gayle
中科院分区：
文献类型：
--
作者：
Ho, Lionel;Hoefel, Daniel;Newcombe, Gayle
导致味道和气味 (T&O) 的化合物，特别是 2-甲基异冰片 (MIB) 和土臭味素，对水务部门来说是一个问题，因为它们难以进行传统的水处理。在这项研究中，生物砂过滤被证明是完全去除 MIB 和土臭素的有效方法，并且去除主要是通过生物降解。此外，在使用来自砂滤器之一的生物膜作为微生物接种物的批量生物反应器实验中，MIB 和土臭味素也被有效降解。在生物反应器实验中，MIB和土臭素的生物降解被确定为伪一级反应，速率常数范围在0.10至0.58 d(-1)之间。当使用 200 和 50 ng l(-1) 的目标浓度时，速率常数取决于微生物接种物的初始浓度，而不取决于 MIB 和土臭味素的初始浓度。此外，当生物膜重新暴露于两种 T&O 化合物时，速率常数会增加。使用 16S rRNA 引导的 PCR-DGGE 进行的富集培养物和随后的群落概况分析鉴定出四种最有可能参与砂滤器和生物反应器内土臭素生物降解的细菌。其中包括假单胞菌属、阿尔法变形菌属、鞘氨醇单胞菌属。和酸杆菌科成员。 (c) 2006 Elsevier Ltd. 保留所有权利。
Taste and odour (T&O) causing compounds, in particular, 2-methylisoborneol (MIB) and geosmin, are a problem for water authorities as they are recalcitrant to conventional water treatment. In this study, biological sand filtration was shown to be an effective process for the complete removal of MIB and geosmin, with removal shown to be predominantly through biodegradation. In addition, MIB and geosmin were also effectively degraded in batch bioreactor experiments using biofilm sourced from one of the sand filters as the microbial inoculum. The biodegradation of MIB and geosmin was determined to be a pseudo-first-order reaction with rate constants ranging between 0.10 and 0.58 d(-1) in the bioreactor experiments. Rate constants were shown to be dependent upon the initial concentration of the microbial inoculum but not the initial concentration of MIB and geosmin when target concentrations of 200 and 50 ng l(-1) were used. Furthermore, rate constants were shown to increase upon re-exposure of the biofilm to both T&O compounds. Enrichment cultures with subsequent community profile analysis using 16S rRNA-directed PCR-DGGE identified four bacteria most likely involved in the biodegradation of geosmin within the sand filters and bioreactors. These included a Pseudomonas sp., Alphaproteobacterium, Sphingomonas sp. and an Acidobacteriaceae member. (c) 2006 Elsevier Ltd. All rights reserved.