Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad
Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad
复制标题
DOI：
10.1016/j.stem.2008.03.005
复制
发表时间：
2008-04-01
期刊：
影响因子：
23.9
通讯作者：
Okano, Hideyuki
中科院分区：
文献类型：
--
作者：
Nagoshi, Narihito;Shibata, Shinsuke;Okano, Hideyuki
虽然最近的报道已经描述了多能的，自我更新的，神经嵴来源的干细胞（NCSC），在各种成年啮齿动物组织中的NCSC还没有得到很好的表征或比较。在此，我们鉴定了骨髓（BM）、背根神经节和须垫中的NCSC，并从编码神经嵴特异性PO-Cre/Floxed-EGFP和Wntl-Cre/Floxed-EGFP的成年转基因小鼠中前瞻性地分离它们。培养的EGFP阳性细胞形成神经球样结构，表达NCSC基因，并能分化为神经元，神经胶质细胞和肌成纤维细胞，但细胞类型的频率是组织来源依赖性的。有趣的是，我们在胚胎阶段的大鼠中肾区域、循环血液和肝脏中观察到了NCSC，这表明NCSC通过血流迁移到BM，并为神经细胞如何从BM产生提供了解释。在可接近的成人组织中鉴定NCSC为神经损伤或疾病后的自体细胞治疗提供了新的潜在来源。
Although recent reports have described multipotent, self-renewing, neural crest-derived stem cells (NCSCs), the NCSCs in various adult rodent tissues have not been well characterized or compared. Here we identified NCSCs in the bone marrow (BM), dorsal root ganglia, and whisker pad and prospectively isolated them from adult transgenic mice encoding neural crest-specific PO-Cre/Floxed-EGFP and Wntl-Cre/Floxed-EGFP. Cultured EGFP-positive cells formed neurosphere-like structures that expressed NCSC genes and could differentiate into neurons, glial cells, and myofibroblasts, but the frequency of the cell types was tissue source dependent. Interestingly, we observed NCSCs in the aortagonad-mesonephros region, circulating blood, and liver at the embryonic stage, suggesting that NCSCs migrate through the bloodstream to the BM and providing an explanation for how neural cells are generated from the BM. The identification of NCSCs in accessible adult tissue provides a new potential source for autologous cell therapy after nerve injury or disease.