Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells
Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells
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DOI：
10.1016/j.yexcr.2011.07.023
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发表时间：
2011-10-15
影响因子：
3.7
通讯作者：
Williams, Elizabeth D.
中科院分区：
文献类型：
--
作者：
Coso, Sanja;Zeng, Yiping;Williams, Elizabeth D.
淋巴管引导间质液体，通过调节白细胞和抗原向淋巴结的运输来调节免疫反应，在癌症环境中使肿瘤细胞能够追踪到区域淋巴结。本研究的目的是确定原代小鼠淋巴管内皮细胞(MLECs)与人淋巴管内皮细胞(HLECs)之间是否存在保守的血管内皮生长因子(VEGF)信号通路。成功地从小鼠真皮和前列腺中分离出LECs。与人晶状体上皮细胞类似，血管内皮细胞生长因子(VEGF)家族配体激活mLECs中的MAPK和PACK信号通路。我们描述了一种分离mLEC的可靠方案，通过利用转基因和基因敲除小鼠模型的力量，将是研究LEC表型如何有助于淋巴管形成和功能改变的有用工具。皇冠版权所有(C)2011由爱思唯尔公司出版。保留所有权利。
Lymphatic vessels guide interstitial fluid, modulate immune responses by regulating leukocyte and antigen trafficking to lymph nodes, and in a cancer setting enable tumor cells to track to regional lymph nodes. The aim of the study was to determine whether primary murine lymphatic endothelial cells (mLECs) show conserved vascular endothelial growth factor (VEGF) signaling pathways with human LECs (hLECs). LECs were successfully isolated from murine dermis and prostate. Similar to hLECs, vascular endothelial growth factor (VEGF) family ligands activated MAPK and pAkt intracellular signaling pathways in mLECs. We describe a robust protocol for isolation of mLECs which, by harnessing the power of transgenic and knockout mouse models, will be a useful tool to study how LEC phenotype contributes to alterations in lymphatic vessel formation and function. Crown Copyright (C) 2011 Published by Elsevier Inc. All rights reserved.