Edaravone, a known free radical scavenger, enhances X-ray-induced apoptosis at low concentrations.
Edaravone, a known free radical scavenger, enhances X-ray-induced apoptosis at low concentrations.
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DOI：
10.1016/j.canlet.2009.12.020
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发表时间：
2010-07
期刊：
影响因子：
9.7
通讯作者：
N. Sasano;A. Enomoto;Y. Hosoi;Y. Katsumura;Y. Matsumoto;A. Morita;K. Shiraishi;K. Miyagawa;H. Igaki;K. Nakagawa
中科院分区：
文献类型：
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作者：
N. Sasano;A. Enomoto;Y. Hosoi;Y. Katsumura;Y. Matsumoto;A. Morita;K. Shiraishi;K. Miyagawa;H. Igaki;K. Nakagawa
据报道，依达拉奉在高浓度下具有辐射防护作用。我们现在报告说，较低剂量的依达拉奉增强了X射线诱导的一些具有p53野生型状态的细胞系的细胞凋亡，例如MOLT-4、Nalm-6和HepG 2。在MOLT-4细胞中使用siRNA敲低p53消除了依达拉奉的放射增敏作用。在依达拉奉存在下，X射线照射后观察到p53丝氨酸15和丝氨酸20磷酸化增强以及p53靶蛋白p53蛋白A的上调。我们的结论是，低剂量的依达拉奉敏化细胞X射线照射通过促进p53依赖的凋亡信号通路。
Edaravone has been reported to have a radioprotective effect at high concentrations. We now report that a lower dose of edaravone enhanced X-ray-induced apoptosis of some cell lines harboring p53 wild-type status, such as MOLT-4, Nalm-6, and HepG2. The knock-down of p53 using siRNA in MOLT-4 cells abolished the radiosensitizing effect of edaravone. Enhanced phosphorylations of p53 at Ser 15 and Ser 20 and up-regulation of PUMA, a p53 target protein, were observed after X-irradiation in the presence of edaravone. We conclude that the low dose of edaravone sensitized cells to X-irradiation by promoting the p53-dependent apoptotic signaling pathway.