Three 2-oxoglutarate-dependent dioxygenase activities of Equisetum arvense L. forming flavone and flavonol from (2S)-naringenin
Three 2-oxoglutarate-dependent dioxygenase activities of Equisetum arvense L. forming flavone and flavonol from (2S)-naringenin
复制标题
DOI：
10.1016/j.phytochem.2011.01.036
复制
发表时间：
2011-05-01
期刊：
影响因子：
3.8
通讯作者：
Martens, Stefan
中科院分区：
文献类型：
--
作者：
Bredebach, Miriam;Matern, Ulrich;Martens, Stefan
Equisetum arvense L.（木贼科马尾草）在不育芽中积累各种黄酮和黄酮醇。对绿色组织粗提物进行的酶测定揭示了查尔酮合酶活性以及与类黄酮生物合成相关的三种其他活性，并被鉴定为黄酮合酶 I、黄烷酮 3 β-羟化酶和黄酮醇合酶。后三种活性因其典型的辅因子需求和特殊的抑制作用而被表征为可溶性、2-氧化戊二酸依赖性双加氧酶。值得注意的是，这是黄酮合酶 I 的首次报道，黄酮合酶 I 被认为仅限于来自遥远植物分类群的伞形科物种。 (C) 2011 Elsevier Ltd. 保留所有权利。
Equisetum arvense L. (Equisetaceae-horsetail) accumulates various flavones and flavonols in infertile shoot. Enzyme assays conducted with crude extracts of the green tissue revealed chalcone synthase activity and also three further activities assigned to flavonoid biosynthesis and identified as flavone synthase I, flavanone 3 beta-hydroxylase and flavonol synthase. The latter three activities were characterized as soluble, 2-oxoglutarate-dependent dioxygenases by their typical cofactor requirements and peculiar inhibition. Notably, this is the first report of flavone synthase I which had been considered to be restricted solely to species of the Apiaceae from a distant plant taxon. (C) 2011 Elsevier Ltd. All rights reserved.